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    Tissue culture and next-generation sequencing: a combined approach for detecting yam (Dioscorea spp.) viruses

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    U18ArtBomerTissueInthomNodev.pdf (2.691Mb)
    Date
    2018
    Author
    Bömer, M.
    Rathnayake, A.I.
    Visendi, P.
    Sewe, S.O.
    Sicat, J.P.A.
    Silva, G.
    Kumar, P.L.
    Seal, S.E.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    In vitro culture offers many advantages for yam germplasm conservation, propagation and international distribution. However, low virus titres in the generated tissues pose a challenge for reliable virus detection, which makes it difficult to ensure that planting material is virus-free. In this study, we evaluated next-generation sequencing (NGS) for virus detection following yam propagation using a robust tissue culture methodology. We detected and assembled the genomes of novel isolates of already characterised viral species of the genera Badnavirus and Potyvirus, confirming the utility of NGS in diagnosing yam viruses and contributing towards the safe distribution of germplasm.
    http://dx.doi.org/10.1016/j.pmpp.2018.06.003
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/2990
    Non-IITA Authors ORCID
    P. Lava Kumarhttps://orcid.org/0000-0003-4388-6510
    Digital Object Identifier (DOI)
    http://dx.doi.org/10.1016/j.pmpp.2018.06.003
    Research Themes
    PLANT PRODUCTION & HEALTH
    IITA Subjects
    Plant Diseases; Plant Health; Plant Production; Tissue Culture; Yam
    Agrovoc Terms
    In Vitro Culture; Rna Sequence; Dioscorea; Mosaic Virus; Tissue Culture; Yams; Virus Free Plants
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Journals
    Physiological and Molecular Plant Pathology
    Collections
    • Journal and Journal Articles4835
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