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Tissue culture and next-generation sequencing: a combined approach for detecting yam (Dioscorea spp.) viruses

dc.contributor.authorBömer, M.
dc.contributor.authorRathnayake, A.I.
dc.contributor.authorVisendi, P.
dc.contributor.authorSewe, S.O.
dc.contributor.authorSicat, J.P.A.
dc.contributor.authorSilva, G.
dc.contributor.authorKumar, P.L.
dc.contributor.authorSeal, S.E.
dc.date.accessioned2019-12-04T11:15:33Z
dc.date.available2019-12-04T11:15:33Z
dc.date.issued2018
dc.identifier.citationBömer, M., Rathnayake, A.I., Visendi, P., Sewe, S.O., Sicat, J.P.A., Silva, G., ... & Seal, S.E. (2018). Tissue culture and next-generation sequencing: a combined approach for detecting yam (Dioscorea spp.) viruses. Physiological and Molecular Plant Pathology, 1-13.
dc.identifier.issn0885-5765
dc.identifier.urihttps://hdl.handle.net/20.500.12478/2990
dc.descriptionArticle purchased; Published online: 19 June 2018
dc.description.abstractIn vitro culture offers many advantages for yam germplasm conservation, propagation and international distribution. However, low virus titres in the generated tissues pose a challenge for reliable virus detection, which makes it difficult to ensure that planting material is virus-free. In this study, we evaluated next-generation sequencing (NGS) for virus detection following yam propagation using a robust tissue culture methodology. We detected and assembled the genomes of novel isolates of already characterised viral species of the genera Badnavirus and Potyvirus, confirming the utility of NGS in diagnosing yam viruses and contributing towards the safe distribution of germplasm.
dc.description.sponsorshipBill & Melinda Gates Foundation
dc.format.extent1-13
dc.language.isoen
dc.subjectIn Vitro Culture
dc.subjectRna Sequence
dc.subjectDioscorea
dc.subjectMosaic Virus
dc.subjectTissue Culture
dc.subjectYams
dc.subjectVirus Free Plants
dc.titleTissue culture and next-generation sequencing: a combined approach for detecting yam (Dioscorea spp.) viruses
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpMaize
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationUniversity of Greenwich
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.creator.identifierP. Lava Kumar: 0000-0003-4388-6510
cg.researchthemePLANT PRODUCTION & HEALTH
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Health
cg.iitasubjectPlant Production
cg.iitasubjectTissue Culture
cg.iitasubjectYam
cg.journalPhysiological and Molecular Plant Pathology
cg.howpublishedFormally Published
cg.accessibilitystatusOpen Access
local.dspaceid94325
cg.targetaudienceScientists
cg.identifier.doihttp://dx.doi.org/10.1016/j.pmpp.2018.06.003


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