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    Polyphasic characterization of rhizobia microsymbionts of common bean [Phaseolus vulgaris (L.)] isolated in Mato Grosso do Sul, a hotspot of Brazilian biodiversity

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    S18ArtCostaPolypyhasicInthomDev.pdf (5.925Mb)
    Date
    2018
    Author
    Costa, M.R.
    Chibeba, A.M.
    Mercante, F.M.
    Hungria, M.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
    Show full item record
    Abstract/Description
    Common bean [Phaseolus vulgaris (Linnaeus)] is the key source of protein, carbohydrates and micronutrients for over 300 million people in the tropics. Like many legumes, P. vulgaris can fix atmospheric nitrogen in symbiosis with rhizobia, alleviating the need for the expensive and polluting N-fertilizers. The crop is known to nodulate with a wide range of rhizobia and, although Brazil is not a center of genetic origin/domestication of P. vulgaris, a variety of rhizobial species have been found as symbionts of the legume. Mato Grosso do Sul (MS) is one of the largest common bean producer states in Brazil, with reports of high yields and abundant natural nodulation. The objective of this study was to evaluate the diversity of 73 indigenous rhizobia isolated from common bean grown in 22 municipalities of MS. Great morphophysiological and genetic diversity was found, as indicated by the six and 35 clusters formed, considering the similarity level of 75 and 70%, respectively, for the phenotypic and rep-PCR dendrograms. Eleven representative isolates were selected for detailed genetic characterization using 16S rRNA and three protein-coding housekeeping genes, glnII, gyrB and recA. We identified species originated from the centers of origin/domestication of the legume, R. etli and R. phaseoli, species probably indigenous of Brazil, R. leucaenae and others of the Rhizobium/Agrobacterium clade, in addition to putative new species. The results highlight the great rhizobial diversity of the region.
    http://dx.doi.org/10.1007/s13199-018-0543-6
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/3200
    Non-IITA Authors ORCID
    Amaral Machaculeha Chibebahttps://orcid.org/0000-0001-6019-4482
    Digital Object Identifier (DOI)
    http://dx.doi.org/10.1007/s13199-018-0543-6
    Research Themes
    BIOTECH & PLANT BREEDING
    IITA Subjects
    Plant Breeding
    Agrovoc Terms
    Biological Nitrogen Fixation; Rhizobium; Common Beans
    Regions
    South America
    Countries
    Brazil
    Journals
    Symbiosis
    Collections
    • Journal and Journal Articles4835
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