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dc.contributor.authorCosta, M.R.
dc.contributor.authorChibeba, A.M.
dc.contributor.authorMercante, F.M.
dc.contributor.authorHungria, M.
dc.date.accessioned2019-12-04T11:18:25Z
dc.date.available2019-12-04T11:18:25Z
dc.date.issued2018
dc.identifier.citationCosta, M.R., Chibeba, A.M., Mercante, F.M. & Hungria, M. (2018). Polyphasic characterization of rhizobia microsymbionts of common bean [Phaseolus vulgaris (L.)] isolated in Mato Grosso do Sul, a hotspot of Brazilian biodiversity. Symbiosis, 1-14.
dc.identifier.issn0334-5114
dc.identifier.urihttps://hdl.handle.net/20.500.12478/3200
dc.descriptionPublished online: 26 Feb 2018
dc.description.abstractCommon bean [Phaseolus vulgaris (Linnaeus)] is the key source of protein, carbohydrates and micronutrients for over 300 million people in the tropics. Like many legumes, P. vulgaris can fix atmospheric nitrogen in symbiosis with rhizobia, alleviating the need for the expensive and polluting N-fertilizers. The crop is known to nodulate with a wide range of rhizobia and, although Brazil is not a center of genetic origin/domestication of P. vulgaris, a variety of rhizobial species have been found as symbionts of the legume. Mato Grosso do Sul (MS) is one of the largest common bean producer states in Brazil, with reports of high yields and abundant natural nodulation. The objective of this study was to evaluate the diversity of 73 indigenous rhizobia isolated from common bean grown in 22 municipalities of MS. Great morphophysiological and genetic diversity was found, as indicated by the six and 35 clusters formed, considering the similarity level of 75 and 70%, respectively, for the phenotypic and rep-PCR dendrograms. Eleven representative isolates were selected for detailed genetic characterization using 16S rRNA and three protein-coding housekeeping genes, glnII, gyrB and recA. We identified species originated from the centers of origin/domestication of the legume, R. etli and R. phaseoli, species probably indigenous of Brazil, R. leucaenae and others of the Rhizobium/Agrobacterium clade, in addition to putative new species. The results highlight the great rhizobial diversity of the region.
dc.format.extent1-14
dc.language.isoen
dc.subjectBiological Nitrogen Fixation
dc.subjectRhizobium
dc.subjectCommon Beans
dc.titlePolyphasic characterization of rhizobia microsymbionts of common bean [Phaseolus vulgaris (L.)] isolated in Mato Grosso do Sul, a hotspot of Brazilian biodiversity
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationUniversidade Estadual de Londrina
cg.contributor.affiliationEmpresa Brasileira de Pesquisa Agropecuaria
cg.contributor.affiliationEmpresa Brasileira de Pesquisa Agropecuaria
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionSouth America
cg.coverage.countryBrazil
cg.creator.identifierAmaral Machaculeha Chibeba: 0000-0001-6019-4482
cg.researchthemeBIOTECH & PLANT BREEDING
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectPlant Breeding
cg.journalSymbiosis
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid94832
cg.targetaudienceScientists
cg.identifier.doihttp://dx.doi.org/10.1007/s13199-018-0543-6


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