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    Agrobacterium mediated transformation of plantain (Musa spp.) cultivar Agbagba

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    U06ArtPillayPloidynNothomNodev.pdf (569.0Kb)
    Date
    2005
    Author
    Tripathi, L.
    Tripathi, J.
    Hughes, J.
    Type
    Journal Article
    Metadata
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    Abstract/Description
    An Agrobacterium-mediated plant transformation system was developed for the production of transgenic plantain [Musa spp. cultivar Agbagba (AAB)]. Apical shoot tips were transformed using Agrobacterium strain EHA105 with the binary vector pCAMBIA 1201, having the hygromycin resistance gene as a selection marker and GUS-INT as a reporter gene. Transient expression of the bglucuronidase (uid A) gene was achieved in transformed apical shoot tips. The hygromycin resistant shoots were regenerated 4 to 5 weeks after co-cultivation of explants with Agrobacterium. The two step selection procedure allowed the regeneration of shoots which were uniformly transformed. The integration of the uid A gene was confirmed by polymerase chain reaction (PCR) and Southern blot analysis. In this study, transformation based on regeneration from apical shoot tips has been demonstrated. This process does not incorporate steps using disorganized cell cultures but uses micropropagation, which has the important advantage that it allows regeneration of homogeneous populations of plants in a short period of time. This study shows the enormous potential for genetic manipulation of Musa species for disease and pest resistance, as well as abiotic factors, using a rapid and non-species specific transformation and regeneration system.
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/3329
    IITA Subjects
    Disease Control; Plantain; Plant Genetic Resources; Research Method; Plant Health; Plant Production; Farm Management; Pests Of Plants; Farming Systems; Food Security; Plant Diseases; Plant Breeding; Livelihoods; Handling, Transport, Storage And Protection Of Agricultural Products
    Agrovoc Terms
    Plantains; Musa; Agrobacterium Mediated Transformation; Apical Shoot Tip; Hygromycin Resistance
    Regions
    Africa; Asia; East Africa; East Asia
    Countries
    Uganda; Taiwan
    Collections
    • Journal and Journal Articles4836
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