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Agrobacterium mediated transformation of plantain (Musa spp.) cultivar Agbagba

dc.contributor.authorTripathi, L.
dc.contributor.authorTripathi, J.
dc.contributor.authorHughes, J.
dc.date.accessioned2019-12-04T11:18:49Z
dc.date.available2019-12-04T11:18:49Z
dc.date.issued2005
dc.identifier.citationTripathi, L., Tripathi, J. & Hughes, J. (2005). Agrobacterium-mediated transformation of plantain (Musa spp.) cultivar Agbagba. African Journal of Biotechnology, 4(12), 1378-1383.
dc.identifier.issn1684-5315
dc.identifier.urihttps://hdl.handle.net/20.500.12478/3329
dc.description.abstractAn Agrobacterium-mediated plant transformation system was developed for the production of transgenic plantain [Musa spp. cultivar Agbagba (AAB)]. Apical shoot tips were transformed using Agrobacterium strain EHA105 with the binary vector pCAMBIA 1201, having the hygromycin resistance gene as a selection marker and GUS-INT as a reporter gene. Transient expression of the bglucuronidase (uid A) gene was achieved in transformed apical shoot tips. The hygromycin resistant shoots were regenerated 4 to 5 weeks after co-cultivation of explants with Agrobacterium. The two step selection procedure allowed the regeneration of shoots which were uniformly transformed. The integration of the uid A gene was confirmed by polymerase chain reaction (PCR) and Southern blot analysis. In this study, transformation based on regeneration from apical shoot tips has been demonstrated. This process does not incorporate steps using disorganized cell cultures but uses micropropagation, which has the important advantage that it allows regeneration of homogeneous populations of plants in a short period of time. This study shows the enormous potential for genetic manipulation of Musa species for disease and pest resistance, as well as abiotic factors, using a rapid and non-species specific transformation and regeneration system.
dc.language.isoen
dc.subjectPlantains
dc.subjectMusa
dc.subjectAgrobacterium Mediated Transformation
dc.subjectApical Shoot Tip
dc.subjectHygromycin Resistance
dc.titleAgrobacterium mediated transformation of plantain (Musa spp.) cultivar Agbagba
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationWorld Vegetable Center
cg.coverage.regionAfrica
cg.coverage.regionAsia
cg.coverage.regionEast Africa
cg.coverage.regionEast Asia
cg.coverage.countryUganda
cg.coverage.countryTaiwan
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectDisease Control
cg.iitasubjectPlantain
cg.iitasubjectPlant Genetic Resources
cg.iitasubjectResearch Method
cg.iitasubjectPlant Health
cg.iitasubjectPlant Production
cg.iitasubjectFarm Management
cg.iitasubjectPests Of Plants
cg.iitasubjectFarming Systems
cg.iitasubjectFood Security
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Breeding
cg.iitasubjectLivelihoods
cg.iitasubjectHandling, Transport, Storage And Protection Of Agricultural Products
cg.accessibilitystatusOpen Access
local.dspaceid95003


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