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    Molecular diversity of Scutellonema bradys populations from Benin, based on ITS1 rDNA and COI mtDNA

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    S18ArtAfohaMolecularInthomDev.pdf (1.581Mb)
    Date
    2018
    Author
    Afoha, S.A.P.E.
    Affokpon, A.
    Waeyenberge, L.
    Sutter, N. de
    Agbangla, C.
    Dansi, A.
    Coyne, D.L.
    Viaene, N.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    In Benin, yam production continues to face numerous production constraints, including yield and quality reduction by Scutellonema bradys. Implementation of efficient management techniques against this pest requires an improved understanding, including at the molecular level, of the pest. The current study aimed at identifying the Scutellonema spp. associated with yam in Benin and investigating the phylogenetic relationships between populations. Nematodes of the genus Scutellonema were obtained from tubers exhibiting external dry rot symptoms. DNA was extracted from nematodes belonging to 138 populations collected from 49 fields from 29 villages. For 51 of these populations, both the ITS1 and COI regions could be amplified via PCR, sequenced, compared with available sequences in the NCBI database and were identified as S. bradys. Maximum likelihood was used to construct 60% consensus phylogenetic trees based on 51 sequences. This phylogenetic analysis did not reveal any genetic separation between populations by cultivar, village, cropping system nor by agroecological zone. Neither could any subgroups within S. bradys be separated, indicating that no subspecies were present. An earlier published species-specific primer set was verified with the DNA of the 51 sequences and was considered a reliable and rapid method for S. bradys identification.
    http://dx.doi.org/10.1007/s40858-018-0221-5
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/3637
    Non-IITA Authors ORCID
    Daniel Coynehttps://orcid.org/0000-0002-2030-6328
    Digital Object Identifier (DOI)
    http://dx.doi.org/10.1007/s40858-018-0221-5
    IITA Subjects
    Plant Production; Yam
    Agrovoc Terms
    Dioscorea; Diagnostics; Yams; Nematodes; Production; Scutellonema Bradys
    Regions
    Africa; West Africa
    Countries
    Benin
    Journals
    Tropical Plant Pathology
    Collections
    • Journal and Journal Articles4835
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