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Molecular diversity of Scutellonema bradys populations from Benin, based on ITS1 rDNA and COI mtDNA

dc.contributor.authorAfoha, S.A.P.E.
dc.contributor.authorAffokpon, A.
dc.contributor.authorWaeyenberge, L.
dc.contributor.authorSutter, N. de
dc.contributor.authorAgbangla, C.
dc.contributor.authorDansi, A.
dc.contributor.authorCoyne, D.L.
dc.contributor.authorViaene, N.
dc.date.accessioned2019-12-04T11:21:01Z
dc.date.available2019-12-04T11:21:01Z
dc.date.issued2018
dc.identifier.citationAfoha, S.A.P.E., Affokpon, A., Waeyenberge, L., de Sutter, N., Agbangla, C., Dansi, A., ... & Viaene, N. (2018). Molecular diversity of Scutellonema bradys populations from Benin, based on ITS1 rDNA and COI mtDNA. Tropical Plant Pathology, 1-10.
dc.identifier.issn1983-2052
dc.identifier.urihttps://hdl.handle.net/20.500.12478/3637
dc.descriptionPublished online: 10 May 2018
dc.description.abstractIn Benin, yam production continues to face numerous production constraints, including yield and quality reduction by Scutellonema bradys. Implementation of efficient management techniques against this pest requires an improved understanding, including at the molecular level, of the pest. The current study aimed at identifying the Scutellonema spp. associated with yam in Benin and investigating the phylogenetic relationships between populations. Nematodes of the genus Scutellonema were obtained from tubers exhibiting external dry rot symptoms. DNA was extracted from nematodes belonging to 138 populations collected from 49 fields from 29 villages. For 51 of these populations, both the ITS1 and COI regions could be amplified via PCR, sequenced, compared with available sequences in the NCBI database and were identified as S. bradys. Maximum likelihood was used to construct 60% consensus phylogenetic trees based on 51 sequences. This phylogenetic analysis did not reveal any genetic separation between populations by cultivar, village, cropping system nor by agroecological zone. Neither could any subgroups within S. bradys be separated, indicating that no subspecies were present. An earlier published species-specific primer set was verified with the DNA of the 51 sequences and was considered a reliable and rapid method for S. bradys identification.
dc.format.extent1-10
dc.language.isoen
dc.subjectDioscorea
dc.subjectDiagnostics
dc.subjectYams
dc.subjectNematodes
dc.subjectProduction
dc.subjectScutellonema Bradys
dc.titleMolecular diversity of Scutellonema bradys populations from Benin, based on ITS1 rDNA and COI mtDNA
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationUniversité d'Abomey Calavi
cg.contributor.affiliationFlanders Research Institute for Agriculture, Fisheries and Food, Belgium
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationGhent University
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryBenin
cg.creator.identifierDaniel Coyne: 0000-0002-2030-6328
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectPlant Production
cg.iitasubjectYam
cg.journalTropical Plant Pathology
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid95716
cg.targetaudienceScientists
cg.identifier.doihttp://dx.doi.org/10.1007/s40858-018-0221-5


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