• Contact Us
    • Send Feedback
    • Login
    View Item 
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    Whole Repository
    CollectionsIssue DateRegionCountryHubAffiliationAuthorsTitlesSubject
    This Sub-collection
    Issue DateRegionCountryHubAffiliationAuthorsTitlesSubject

    My Account

    Login

    Welcome to the International Institute of Tropical Agriculture Research Repository

    What would you like to view today?

    Identification of RAPD markers linked to A and B genome sequences in Musa L

    Thumbnail
    View/Open
    pillay-identification-2000.pdf (1.600Mb)
    Date
    2000
    Author
    Pillay, M.
    Nwakanma, D.
    Tenkouano, A.
    Type
    Journal Article
    Metadata
    Show full item record
    Abstract/Description
    Plantains and bananas (Musa spp. sect. eumusa) originated from intra- and interspecific hybridization between two wild diploid species, M. acuminata Colla. and M. balbisiana Colla., which contributed the A and B genomes, respectively. Polyploidy and hybridization have given rise to a number of diploid, triploid, and tetraploid clones with different permutations of the A and B genomes. Thus, dessert and highland bananas are classified mainly as AAA, plantains are AAB, and cooking bananas are ABB. Classification of Musa into genomic groups has been based on morphological characteristics. This study aimed to identify RAPD (random amplified polymorphic DNA) markers for the A and B genomes. Eighty 10-mer Operon primers were used to amplify DNA from M. acuminata subsp. burmannicoides clone 'Calcutta 4' (AA genomes) and M. balbisiana clone 'Honduras' (BB genomes). Three primers (A17, A18, and D10) that produced unique genome-specific fragments in the two species were identified. These primers were tested in a sample of 40 genotypes representing various genome combinations. The RAPD markers were able to elucidate the genome composition of all the genotypes. The results showed that RAPD analysis can provide a quick and reliable system for genome identification in Musa that could facilitate genome characterization and manipulations in breeding lines.Key words: banana and plantain, A and B genomes, genomic groups, RAPD markers.
    https://doi.org/10.1139/g00-038
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/3705
    Digital Object Identifier (DOI)
    https://doi.org/10.1139/g00-038
    IITA Subjects
    Genetic Improvement; Banana; Diseases Control; Livelihoods; Plant Breeding; Farm Management; Pests Of Plants; Plant Diseases; Crop Husbandry; Handling, Transport, Storage And Protection Of Agricultural Products; Food Security; Plant Genetic Resources; Plant Health; Plant Production; Plantain; Agribusiness
    Agrovoc Terms
    Genomic Groups; Rapd Makers; Banana And Plantain; A And B Genomes; Genotypes; Chromosomes; M.Acuminata Colla. And M. Balbisiana Colla; Hybridization
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Collections
    • Journal and Journal Articles4839
    copyright © 2019  IITASpace. All rights reserved.
    IITA | Open Access Repository