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Identification of RAPD markers linked to A and B genome sequences in Musa L

dc.contributor.authorPillay, M.
dc.contributor.authorNwakanma, D.
dc.contributor.authorTenkouano, A.
dc.date.accessioned2019-12-04T11:21:12Z
dc.date.available2019-12-04T11:21:12Z
dc.date.issued2000
dc.identifier.citationPillay, M., Nwakanma, D. & Tenkouano, A. (2000). Identification of RAPD markers linked to A and B genome sequences in Musa L. Genome, 43, 763-767.
dc.identifier.urihttps://hdl.handle.net/20.500.12478/3705
dc.description.abstractPlantains and bananas (Musa spp. sect. eumusa) originated from intra- and interspecific hybridization between two wild diploid species, M. acuminata Colla. and M. balbisiana Colla., which contributed the A and B genomes, respectively. Polyploidy and hybridization have given rise to a number of diploid, triploid, and tetraploid clones with different permutations of the A and B genomes. Thus, dessert and highland bananas are classified mainly as AAA, plantains are AAB, and cooking bananas are ABB. Classification of Musa into genomic groups has been based on morphological characteristics. This study aimed to identify RAPD (random amplified polymorphic DNA) markers for the A and B genomes. Eighty 10-mer Operon primers were used to amplify DNA from M. acuminata subsp. burmannicoides clone 'Calcutta 4' (AA genomes) and M. balbisiana clone 'Honduras' (BB genomes). Three primers (A17, A18, and D10) that produced unique genome-specific fragments in the two species were identified. These primers were tested in a sample of 40 genotypes representing various genome combinations. The RAPD markers were able to elucidate the genome composition of all the genotypes. The results showed that RAPD analysis can provide a quick and reliable system for genome identification in Musa that could facilitate genome characterization and manipulations in breeding lines.Key words: banana and plantain, A and B genomes, genomic groups, RAPD markers.
dc.description.sponsorshipDirectorate-General for Development Cooperation and Humanitarian Aid, Belgium
dc.language.isoen
dc.subjectGenomic Groups
dc.subjectRapd Makers
dc.subjectBanana And Plantain
dc.subjectA And B Genomes
dc.subjectGenotypes
dc.subjectChromosomes
dc.subjectM.Acuminata Colla. And M. Balbisiana Colla
dc.subjectHybridization
dc.titleIdentification of RAPD markers linked to A and B genome sequences in Musa L
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.isijournalISI Journal
cg.authorship.typesCGIAR single centre
cg.iitasubjectGenetic Improvement
cg.iitasubjectBanana
cg.iitasubjectDiseases Control
cg.iitasubjectLivelihoods
cg.iitasubjectPlant Breeding
cg.iitasubjectFarm Management
cg.iitasubjectPests Of Plants
cg.iitasubjectPlant Diseases
cg.iitasubjectCrop Husbandry
cg.iitasubjectHandling, Transport, Storage And Protection Of Agricultural Products
cg.iitasubjectFood Security
cg.iitasubjectPlant Genetic Resources
cg.iitasubjectPlant Health
cg.iitasubjectPlant Production
cg.iitasubjectPlantain
cg.iitasubjectAgribusiness
cg.accessibilitystatusOpen Access
local.dspaceid95860
cg.identifier.doihttps://doi.org/10.1139/g00-038


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