• Contact Us
    • Send Feedback
    • Login
    View Item 
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    Whole Repository
    CollectionsIssue DateRegionCountryHubAffiliationAuthorsTitlesSubject
    This Sub-collection
    Issue DateRegionCountryHubAffiliationAuthorsTitlesSubject

    My Account

    Login

    Welcome to the International Institute of Tropical Agriculture Research Repository

    What would you like to view today?

    Cowpea (Vigna unguiculata L. Walp) hosts several widespread bradyrhizobial root nodule symbionts across contrasting agro-ecological production areas in Kenya

    Thumbnail
    View/Open
    U18ArtNdunguCowpeaInthomNodev.pdf (931.8Kb)
    Date
    2018-07-01
    Author
    Ndungu, S.M.
    Messmer, M.M.
    Ziegler, D.
    Gamper, H.A.
    Mészáros, E.
    Thuita, M.
    Vanlauwe, Bernard
    Frossard, E.
    Thonar, C.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
    Show full item record
    Abstract/Description
    Cowpea (Vigna unguiculata L. Walp.) is an important African food legume suitable for dry regions. It is the main legume in two contrasting agro-ecological regions of Kenya as an important component of crop rotations because of its relative tolerance to unpredictable drought events. This study was carried out in an effort to establish a collection of bacterial root nodule symbionts and determine their relationship to physicochemical soil parameters as well as any geographical distributional patterns. Bradyrhizobium spp. were found to be widespread in this study and several different types could be identified at each site. Unique but rare symbionts were recovered from the nodules of plants sampled in a drier in-land region, where there were also overall more different bradyrhizobia found. Plants raised in soil from uncultivated sites with a natural vegetation cover tended to also associate with more different bradyrizobia. The occurrence and abundance of different bradyrhizobia correlated with differences in soil texture and pH, but did neither with the agro-ecological origin, nor the origin from cultivated (n = 15) or uncultivated (n = 5) sites. The analytical method, protein profiling of isolated strains by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS), provided higher resolution than 16S rRNA gene sequencing and was applied in this study for the first time to isolates recovered directly from field-collected cowpea root nodules. The method thus seems suitable for screening isolate collections on the presence of different groups, which, provided an appropriate reference database, can also be assigned to known species.
    http://dx.doi.org/10.1016/j.agee.2017.12.014
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/3847
    Non-IITA Authors ORCID
    Moses Thuitahttps://orcid.org/0000-0002-6731-9492
    bernard vanlauwehttps://orcid.org/0000-0001-6016-6027
    Digital Object Identifier (DOI)
    http://dx.doi.org/10.1016/j.agee.2017.12.014
    Research Themes
    NATURAL RESOURCE MANAGEMENT
    IITA Subjects
    Cowpea; Grain Legumes; Natural Resource Management
    Agrovoc Terms
    Bradyrhizobium; Distribution; Cowpeas; Vigna Unguiculata; Agroecology; Root Nodules
    Regions
    Africa; East Africa
    Countries
    Kenya
    Journals
    Agriculture, Ecosystems & Environment
    Collections
    • Journal and Journal Articles4835
    copyright © 2019  IITASpace. All rights reserved.
    IITA | Open Access Repository