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Cowpea (Vigna unguiculata L. Walp) hosts several widespread bradyrhizobial root nodule symbionts across contrasting agro-ecological production areas in Kenya

dc.contributor.authorNdungu, S.M.
dc.contributor.authorMessmer, M.M.
dc.contributor.authorZiegler, D.
dc.contributor.authorGamper, H.A.
dc.contributor.authorMészáros, E.
dc.contributor.authorThuita, M.
dc.contributor.authorVanlauwe, Bernard
dc.contributor.authorFrossard, E.
dc.contributor.authorThonar, C.
dc.date.accessioned2019-12-04T11:21:40Z
dc.date.available2019-12-04T11:21:40Z
dc.date.issued2018-07-01
dc.identifier.citationNdungu, S.M., Messmer, M.M., Ziegler, D., Gamper, H.A., Mészáros, É., Thuita, M., ... & Thonar, C. (2018). Cowpea (Vigna unguiculata L. Walp) hosts several widespread bradyrhizobial root nodule symbionts across contrasting agro-ecological production areas in Kenya. Agriculture, Ecosystems & Environment, 261, 161-171.
dc.identifier.issn0167-8809
dc.identifier.urihttps://hdl.handle.net/20.500.12478/3847
dc.descriptionArticle purchased; Published online: 3 Feb 2018
dc.description.abstractCowpea (Vigna unguiculata L. Walp.) is an important African food legume suitable for dry regions. It is the main legume in two contrasting agro-ecological regions of Kenya as an important component of crop rotations because of its relative tolerance to unpredictable drought events. This study was carried out in an effort to establish a collection of bacterial root nodule symbionts and determine their relationship to physicochemical soil parameters as well as any geographical distributional patterns. Bradyrhizobium spp. were found to be widespread in this study and several different types could be identified at each site. Unique but rare symbionts were recovered from the nodules of plants sampled in a drier in-land region, where there were also overall more different bradyrhizobia found. Plants raised in soil from uncultivated sites with a natural vegetation cover tended to also associate with more different bradyrizobia. The occurrence and abundance of different bradyrhizobia correlated with differences in soil texture and pH, but did neither with the agro-ecological origin, nor the origin from cultivated (n = 15) or uncultivated (n = 5) sites. The analytical method, protein profiling of isolated strains by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS), provided higher resolution than 16S rRNA gene sequencing and was applied in this study for the first time to isolates recovered directly from field-collected cowpea root nodules. The method thus seems suitable for screening isolate collections on the presence of different groups, which, provided an appropriate reference database, can also be assigned to known species.
dc.description.sponsorshipBill & Melinda Gates Foundation
dc.format.extent161-171
dc.language.isoen
dc.subjectBradyrhizobium
dc.subjectDistribution
dc.subjectCowpeas
dc.subjectVigna Unguiculata
dc.subjectAgroecology
dc.subjectRoot Nodules
dc.titleCowpea (Vigna unguiculata L. Walp) hosts several widespread bradyrhizobial root nodule symbionts across contrasting agro-ecological production areas in Kenya
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpMaize
cg.contributor.crpGrain Legumes
cg.contributor.affiliationInstitute of Agricultural Sciences, Switzerland
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationResearch Institute of Organic Agriculture, Switzerland
cg.contributor.affiliationMabritec AG, Switzerland
cg.contributor.affiliationUniversité de Liège
cg.coverage.regionAfrica
cg.coverage.regionEast Africa
cg.coverage.countryKenya
cg.creator.identifierMoses Thuita: 0000-0002-6731-9492
cg.creator.identifierbernard vanlauwe: 0000-0001-6016-6027
cg.researchthemeNATURAL RESOURCE MANAGEMENT
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectCowpea
cg.iitasubjectGrain Legumes
cg.iitasubjectNatural Resource Management
cg.journalAgriculture, Ecosystems & Environment
cg.howpublishedFormally Published
cg.accessibilitystatusOpen Access
local.dspaceid96136
cg.targetaudienceScientists
cg.identifier.doihttp://dx.doi.org/10.1016/j.agee.2017.12.014


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