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    A method for generating virus-free cassava plants to combat viral disease epidemics in Africa

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    U18ArtMaruthiMethodInthomDev.pdf (1.960Mb)
    Date
    2019
    Author
    Maruthi, M.N.
    Whitfield, E.C.
    Otti, G.
    Tumwegamire, S.
    Kanju, E.
    Legg, J.P.
    Mkamilo, G.
    Kawuki, R.
    Benesi, I.
    Zacarias, A.
    Munga, T.
    Mwatuni, F.
    Mbugua, E.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    Here, we report a method to clean cassava plants from viral infections that cause cassava mosaic and brown streak diseases in Africa. Infected plants of resistant or tolerant varieties from Malawi, Mozambique, Kenya, Tanzania and Uganda were cleaned in the UK using a combination of tissue culture, chemotherapy and thermotherapy. In the first cycle of our virus-indexing procedure, we successfully cleaned 27 of the 31 varieties (87%), and after an additional three cleaning cycles, all plants were virus-free. Virus-free tissue-cultured plants were shipped back to Africa for distribution to farmers. This first cross-boundary effort provides important lessons for mitigating the two-major cassava viral diseases.
    http://dx.doi.org/10.1016/j.pmpp.2018.09.002
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/4934
    Non-IITA Authors ORCID
    Silver Tumwegamirehttps://orcid.org/0000-0003-2820-6337
    Edward Kanjuhttps://orcid.org/0000-0002-0413-1302
    James Legghttps://orcid.org/0000-0003-4140-3757
    Digital Object Identifier (DOI)
    http://dx.doi.org/10.1016/j.pmpp.2018.09.002
    Research Themes
    BIOTECH & PLANT BREEDING; PLANT PRODUCTION & HEALTH
    IITA Subjects
    Cassava; Plant Breeding; Plant Health; Plant Production
    Agrovoc Terms
    Cassava; Manihot Esculenta; Cassava Mosaic Virus; Virus Indexing; East Africa; Southern Africa
    Regions
    Africa; East Africa; Southern Africa
    Countries
    Kenya; Malawi; Mozambique; Tanzania; Uganda
    Journals
    Physiological and Molecular Plant Pathology
    Collections
    • Journal and Journal Articles4835
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