• Contact Us
    • Send Feedback
    • Login
    View Item 
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    Whole Repository
    CollectionsIssue DateRegionCountryHubAffiliationAuthorsTitlesSubject
    This Sub-collection
    Issue DateRegionCountryHubAffiliationAuthorsTitlesSubject

    My Account

    Login

    Welcome to the International Institute of Tropical Agriculture Research Repository

    What would you like to view today?

    Phenotypic, host range and symbiotic characteristics of indigenous soybean nodulating rhizobia from Ethiopian soils

    Thumbnail
    View/Open
    U18ArtAberaPhenotypicNothomDev.pdf (862.3Kb)
    Date
    2018
    Author
    Abera, Y.
    Masso, C.
    Assefa, F.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
    Show full item record
    Abstract/Description
    Soybean is an exotic crop to Ethiopia and may not necessarily have a specific endosymbiont in the soil. However, since it is a promiscuous host, nodulated by cross nodulating rhizobia, it is likely that some compatible endosymbionts exist from heterologous hosts that could nodulate it with effective nitrogen fixation. This necessitated the search for effective indigenous rhizobia isolates and/or compatible and effective cross-inoculating rhizobia that are already adapted to local conditions. To this end, a total of 67 bacterial isolates were trapped from different soil samples using two soybean varieties (Clark-63K and Awassa-95) and one cowpea variety (Bole), to evaluate their diversity and screen for their symbiotic effectiveness. Accordingly, the majority of isolates (93%) were tentatively categorized into alkali producing slow growing Bradyrhiobium spp. and the others (7%) were fast growing and acid producing rhizobia. The isolates showed differences in utilizing various carbon and nitrogen sources and tolerance to acidity, salinity and temperature. The isolates were also diverse in their inherent antibiotic and heavy metal resistance. All the isolates were able to nodulate soybean variety Clark-63K with significant difference in their capacity to infect and effectively fix nitrogen evidenced from variations in nodulation parameters and shoot dry weights. Accordingly, the isolates induced nodulation with nodule number ranging from 2 to 49 nodules plant-1; nodule dry weight of 16 mg plant-1 to 94 mg plant-1 and shoot dry weight between 585 and 1012 mg plant-1. Using shoot dry weight as an indicator of the relative effectiveness of the isolates, 12% of the isolates were highly effective (SE > 80%) and 88% were effective (SE from 50 to 80%) on soybean. Furthermore, the isolates showed narrow and broad host ranges on four legume species viz., cowpea, mung bean, pigeon pea, and peanut. Accordingly, many isolates (67%) formed nodules with effective nitrogen fixation with cowpea (Vigna unguiculata), pigeon pea (Cajanus cajan) (47%), and on few cases with mung bean (Vigna radiata) showing different level of effectiveness. However, the data showed very narrow host range on peanut (Arachis hypogaea) where, only one isolate formed effective nodules.
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/4936
    Non-IITA Authors ORCID
    Cargele Massohttps://orcid.org/0000-0002-3980-6832
    Research Themes
    NATURAL RESOURCE MANAGEMENT; BIOTECH & PLANT BREEDING
    IITA Subjects
    Grain Legumes; Natural Resource Management; Plant Breeding; Soybean
    Agrovoc Terms
    Soybeans; Grain Legumes; Bradyrhiobium; Varieties; Ethiopia; East Africa; Phenotypic Variation
    Regions
    Africa; East Africa
    Countries
    Ethiopia
    Journals
    Ethiopian Journal of Agricultural Sciences
    Collections
    • Journal and Journal Articles4460
    copyright © 2019  IITASpace. All rights reserved.
    IITA | Open Access Repository