Show simple item record

dc.contributor.authorAbera, Y.
dc.contributor.authorMasso, C.
dc.contributor.authorAssefa, F.
dc.date.accessioned2019-12-04T11:27:26Z
dc.date.available2019-12-04T11:27:26Z
dc.date.issued2018
dc.identifier.citationAbera, Y., Massa, C. & Assefa, F. (2018). Phenotypic, host range and symbiotic characteristics of indigenous soybean nodulating rhizobia from Ethiopian soils. Ethiopian Journal of Agricultural Sciences, 28(3), 95-116.
dc.identifier.issn2415-2382
dc.identifier.urihttps://hdl.handle.net/20.500.12478/4936
dc.descriptionOpen Access Article
dc.description.abstractSoybean is an exotic crop to Ethiopia and may not necessarily have a specific endosymbiont in the soil. However, since it is a promiscuous host, nodulated by cross nodulating rhizobia, it is likely that some compatible endosymbionts exist from heterologous hosts that could nodulate it with effective nitrogen fixation. This necessitated the search for effective indigenous rhizobia isolates and/or compatible and effective cross-inoculating rhizobia that are already adapted to local conditions. To this end, a total of 67 bacterial isolates were trapped from different soil samples using two soybean varieties (Clark-63K and Awassa-95) and one cowpea variety (Bole), to evaluate their diversity and screen for their symbiotic effectiveness. Accordingly, the majority of isolates (93%) were tentatively categorized into alkali producing slow growing Bradyrhiobium spp. and the others (7%) were fast growing and acid producing rhizobia. The isolates showed differences in utilizing various carbon and nitrogen sources and tolerance to acidity, salinity and temperature. The isolates were also diverse in their inherent antibiotic and heavy metal resistance. All the isolates were able to nodulate soybean variety Clark-63K with significant difference in their capacity to infect and effectively fix nitrogen evidenced from variations in nodulation parameters and shoot dry weights. Accordingly, the isolates induced nodulation with nodule number ranging from 2 to 49 nodules plant-1; nodule dry weight of 16 mg plant-1 to 94 mg plant-1 and shoot dry weight between 585 and 1012 mg plant-1. Using shoot dry weight as an indicator of the relative effectiveness of the isolates, 12% of the isolates were highly effective (SE > 80%) and 88% were effective (SE from 50 to 80%) on soybean. Furthermore, the isolates showed narrow and broad host ranges on four legume species viz., cowpea, mung bean, pigeon pea, and peanut. Accordingly, many isolates (67%) formed nodules with effective nitrogen fixation with cowpea (Vigna unguiculata), pigeon pea (Cajanus cajan) (47%), and on few cases with mung bean (Vigna radiata) showing different level of effectiveness. However, the data showed very narrow host range on peanut (Arachis hypogaea) where, only one isolate formed effective nodules.
dc.format.extent95-116
dc.language.isoen
dc.rightsCC-BY-4.0
dc.subjectSoybeans
dc.subjectGrain Legumes
dc.subjectBradyrhiobium
dc.subjectVarieties
dc.subjectEthiopia
dc.subjectEast Africa
dc.subjectPhenotypic Variation
dc.titlePhenotypic, host range and symbiotic characteristics of indigenous soybean nodulating rhizobia from Ethiopian soils
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpGrain Legumes and Dryland Cereals
cg.contributor.affiliationEthiopian Institute of Agricultural Research, Debrezeit Agricultural Research Center, Ethiopia
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationAddis Ababa University
cg.coverage.regionAfrica
cg.coverage.regionEast Africa
cg.coverage.countryEthiopia
cg.creator.identifierCargele Masso: 0000-0002-3980-6832
cg.researchthemeNATURAL RESOURCE MANAGEMENT
cg.researchthemeBIOTECH & PLANT BREEDING
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectGrain Legumes
cg.iitasubjectNatural Resource Management
cg.iitasubjectPlant Breeding
cg.iitasubjectSoybean
cg.journalEthiopian Journal of Agricultural Sciences
cg.howpublishedFormally Published
cg.accessibilitystatusOpen Access
local.dspaceid101912
cg.targetaudienceScientists


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record