Show simple item record

EST-SSR development for Digitaria exilis and its relatives D. iburua and D. longiflora from transcriptome sequences

dc.contributor.authorOlodo, K.F.
dc.contributor.authorGueye, M.C.
dc.contributor.authorCalatayud, C.
dc.contributor.authorDiop, B.M.
dc.contributor.authorKane, N.A.
dc.contributor.authorNgom, A.
dc.contributor.authorNtui, V.O.
dc.contributor.authorBarreto, M.M.S.
dc.contributor.authorUyoh, E.A.
dc.contributor.authorAbraham, S.
dc.contributor.authorVigouroux, Y.
dc.contributor.authorBillot, C.
dc.contributor.authorBarnaud, A.
dc.date.accessioned2019-12-04T11:30:10Z
dc.date.available2019-12-04T11:30:10Z
dc.date.issued2018
dc.identifier.citationOlodo, K.F., Gueye, M.C., Calatayud, C., Diop, B.M., Kane, N.A., Ngom, A., ... & Vigouroux, Y. (2018). EST-SSR development for Digitaria exilis and its relatives D. iburua and D. longiflora from transcriptome sequences. Plant Genetic Resources, 1-5.
dc.identifier.issn1479-2621
dc.identifier.urihttps://hdl.handle.net/20.500.12478/5211
dc.descriptionPublished online: 21 Nov 2018
dc.description.abstractDigitaria exilis is an important indigenous cereal in West Africa. The first fonio reference transcriptome was released and became a key tool for developing new molecular markers contributing to a better understanding of its genetic diversity. A total of 126 new putative primer pairs were successfully designed in 37,327 unigenes from the D. exilis transcriptome. Thirty-seven primer pairs were randomly selected and tested for their ability to cross-amplify to related species. Clear amplification patterns were observed on 24 primer pairs. Of these, 71, 74 and 35% showed polymorphism in three species: D. exilis, D. longiflora and D. iburua. The transferability from D. exilis was 96% to D. longiflora and 71% to D. iburua. The new SSR markers confirmed the close genetic proximity of D. exilis with D. longiflora and its stronger genetic difference of D. exilis from D. iburua. These markers will be valuable for completing future knowledge on Digitaria evolutionary history, and for testing gene flows between related species.
dc.description.sponsorshipGerman Academic Exchange Service
dc.format.extent1-5
dc.language.isoen
dc.rightsCC-BY-4.0
dc.subjectDigitaria
dc.subjectMicrosatellites
dc.subjectPoaceae
dc.subjectWest Africa
dc.titleEST-SSR development for Digitaria exilis and its relatives D. iburua and D. longiflora from transcriptome sequences
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationInstitut de Recherche pour le Développement
cg.contributor.affiliationInstitut Sénégalais de Recherches Agricoles
cg.contributor.affiliationUniversité Cheikh Anta Diop
cg.contributor.affiliationCentre de Coopération Internationale en Recherche Agronomique pour le Développement
cg.contributor.affiliationUniversité de Montpellier
cg.contributor.affiliationLaboratoire Mixte International Adaptation des Plantes et microorganismes associés aux Stress Environnementaux, Senegal
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationUniversity of Calabar
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryBenin
cg.coverage.countryBurkina Faso
cg.coverage.countryGuinea
cg.coverage.countryMali
cg.coverage.countryNiger
cg.coverage.countrySenegal
cg.coverage.countryTogo
cg.creator.identifierValentine Ntui: 0000-0003-4709-8087
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.journalPlant Genetic Resources
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid102896
cg.targetaudienceScientists
cg.identifier.doihttp://dx.doi.org/10.1017/s147926211800040


Files in this item

Thumbnail
Name:
S18ArtOlodoDevelopmentInthomDev.pdf
Size:
145.0Kb
Format:
PDF
View/Open

This item appears in the following Collection(s)

Show simple item record