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    One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins

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    Date
    2000
    Author
    Machuka, J.
    Okeola, O.
    Type
    Journal Article
    Metadata
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    Abstract/Description
    Seed proteins were extracted from the African yam bean (AYB; Sphenostylis stenocarpa), an underutilized West African food legume. One- and two-dimensional polyacrylamide gel electrophoresis was then used to analyze the albumin fraction, galactose-specific lectins purified on immobilized galactose−Sepharose 4B, and abundant non-lectin seed proteins left over following affinity chromatography. N-terminal sequencing of prominently resolved polypetide bands led to identification of proteins having sequence homology with characterized legume seed proteins, namely, mung bean seed albumin, pea α-fucosidase, soybean Kunitz-type trypsin inhibitor, an endochitinase, pea pathogenesis-related protein, and/or cowpea seed storage proteins. Minor lectin-like proteins lacking hemagglutinating activity against rabbit and human erythrocytes were also identified. Because proteins such as protease inhibitors, chitinases, pathogenesis-related proteins, and lectins are known to have antimetabolic effects, the findings from this study may have relevance in the acceptability, adoption, and utilization of AYB as human food.
    https://doi.org/10.1021/jf990800x
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/5292
    Digital Object Identifier (DOI)
    https://doi.org/10.1021/jf990800x
    IITA Subjects
    Grain Legumes; Food Security; Food Systems; Plant Diseases
    Agrovoc Terms
    Legumes; Foods; Proteins; Pathogenesis
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Collections
    • Journal and Journal Articles4835
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