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One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins

dc.contributor.authorMachuka, J.
dc.contributor.authorOkeola, O.
dc.date.accessioned2019-12-04T11:30:27Z
dc.date.available2019-12-04T11:30:27Z
dc.date.issued2000
dc.identifier.citationMachuka, J. & Okeola, O. (2000). One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins. Journal of Agricultural and Food Chemistry, 48(6), 2296-2299.
dc.identifier.urihttps://hdl.handle.net/20.500.12478/5292
dc.description.abstractSeed proteins were extracted from the African yam bean (AYB; Sphenostylis stenocarpa), an underutilized West African food legume. One- and two-dimensional polyacrylamide gel electrophoresis was then used to analyze the albumin fraction, galactose-specific lectins purified on immobilized galactose−Sepharose 4B, and abundant non-lectin seed proteins left over following affinity chromatography. N-terminal sequencing of prominently resolved polypetide bands led to identification of proteins having sequence homology with characterized legume seed proteins, namely, mung bean seed albumin, pea α-fucosidase, soybean Kunitz-type trypsin inhibitor, an endochitinase, pea pathogenesis-related protein, and/or cowpea seed storage proteins. Minor lectin-like proteins lacking hemagglutinating activity against rabbit and human erythrocytes were also identified. Because proteins such as protease inhibitors, chitinases, pathogenesis-related proteins, and lectins are known to have antimetabolic effects, the findings from this study may have relevance in the acceptability, adoption, and utilization of AYB as human food.
dc.language.isoen
dc.subjectLegumes
dc.subjectFoods
dc.subjectProteins
dc.subjectPathogenesis
dc.titleOne and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.isijournalISI Journal
cg.authorship.typesCGIAR single centre
cg.iitasubjectGrain Legumes
cg.iitasubjectFood Security
cg.iitasubjectFood Systems
cg.iitasubjectPlant Diseases
cg.accessibilitystatusLimited Access
local.dspaceid103649
cg.identifier.doihttps://doi.org/10.1021/jf990800x


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