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    Genetic transformation of banana and plantain (Musa spp.) via particle bombardment

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    Date
    1995
    Author
    Sági, L.
    Panis, B.
    Remy, S.
    Schoofs, H.
    Smet, K. de
    Swennen, R.
    Cammue, B.P.
    Type
    Journal Article
    Metadata
    Show full item record
    Abstract/Description
    We have developed a simple protocol to allow the production of transgenic banana plants. Foreign genes were delivered into embryogenic suspension cells using accelerated particles coated with DNA. Bombardment parameters were optimized for a modified particle gun resulting in high levels of transient expression of the β-glucuronidase gene in both banana and plantain cells. Bombarded banana cells were selected with hygromycin and regenerated into plants. Molecular and histochemical characterization of transformants revealed the stable integration of the transferred genes into the banana genome.
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/5774
    IITA Subjects
    Food Security; Banana; Plantain; Genetic Improvement
    Agrovoc Terms
    Bananas; Plantains; Genes
    Regions
    Acp; Africa; Europe; West Africa
    Countries
    Belgium; Nigeria
    Collections
    • Journal and Journal Articles4835
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