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dc.contributor.authorSági, L.
dc.contributor.authorPanis, B.
dc.contributor.authorRemy, S.
dc.contributor.authorSchoofs, H.
dc.contributor.authorSmet, K. de
dc.contributor.authorSwennen, R.
dc.contributor.authorCammue, B.P.
dc.date.accessioned2019-12-04T11:33:41Z
dc.date.available2019-12-04T11:33:41Z
dc.date.issued1995
dc.identifier.citationSagi, L., Panis, B., Remy, S., Schoofs, H., De Smet, K., Swennen, R. & Cammue, B.P. (1995). Genetic transformation of banana and plantain (Musa spp.) via particle bombardment. Nature Biotechnology, 13, 481-485.
dc.identifier.urihttps://hdl.handle.net/20.500.12478/5774
dc.description.abstractWe have developed a simple protocol to allow the production of transgenic banana plants. Foreign genes were delivered into embryogenic suspension cells using accelerated particles coated with DNA. Bombardment parameters were optimized for a modified particle gun resulting in high levels of transient expression of the β-glucuronidase gene in both banana and plantain cells. Bombarded banana cells were selected with hygromycin and regenerated into plants. Molecular and histochemical characterization of transformants revealed the stable integration of the transferred genes into the banana genome.
dc.language.isoen
dc.subjectBananas
dc.subjectPlantains
dc.subjectGenes
dc.titleGenetic transformation of banana and plantain (Musa spp.) via particle bombardment
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationKatholieke Universiteit, Leuven
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAcp
cg.coverage.regionAfrica
cg.coverage.regionEurope
cg.coverage.regionWest Africa
cg.coverage.countryBelgium
cg.coverage.countryNigeria
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectFood Security
cg.iitasubjectBanana
cg.iitasubjectPlantain
cg.iitasubjectGenetic Improvement
cg.accessibilitystatusLimited Access
local.dspaceid105054


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