• Contact Us
    • Send Feedback
    • Login
    View Item 
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    Whole Repository
    CollectionsIssue DateRegionCountryHubAffiliationAuthorsTitlesSubject
    This Sub-collection
    Issue DateRegionCountryHubAffiliationAuthorsTitlesSubject

    My Account

    Login

    Welcome to the International Institute of Tropical Agriculture Research Repository

    What would you like to view today?

    Detached shoot technique to evaluate the reaction of soybean cultivars to Sclerotium rolfsii

    Thumbnail
    Date
    1991
    Author
    Akem, C.
    Dashiell, Kenton E.
    Type
    Journal Article
    Metadata
    Show full item record
    Abstract/Description
    Shoot-tips 15cm long from 15 soybean cultivars and breeding lines were individually immersed in Hoagland's solution in 1 × 14 cm test tubes, and supported by cotton plugs. All leaves were removed leaving about 1 cm of each petiole on the shoot. A 4 mm mycelial plug of Sclerotium rolfsii Sacc., taken from the periphery of a 3-day-old culture grown on acidified potato dextrose agar (PDA) media was placed between the stem and a petiole in the middle of the shoot. Tubes with shoots were then placed in a polyethylene enclosure in a growth room where the day and night temperatures averaged 31 ± 2°C and 24 ± 2°C, respectively. Relative humidity (r.h.) was maintained at 80–90% by lining the bottom of the enclosure with wet burlap. Lesions appeared on shoot tips 2 days after inoculation, and their length was measured 2, 3 and 4 days later. On three cultivars, TG × 1436-1 E, TG × 1596-2E and TG × 1614-1E, the rate of lesion expansion was significantly less than that on the other cultivars. One week after inoculation, tubes were drained, and shoots left in the chamber at 50–60% r.h. to allow sclerotia to form. Sclerotia from each shoot were collected. The cultivars TG × 1436-1 D and TG × 1596-2E produced the fewest sclerotia per shoot-tip. Sclerotial viability, determined by germination on PDA at 25 ± 2°C in darkness, ranged from 38 to 99%. This method is effective in differentiating reactions of soybean cultivars to S. rolfsii.
    https://doi.org/10.1016/0261-2194(91)90014-I
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/5975
    Digital Object Identifier (DOI)
    https://doi.org/10.1016/0261-2194(91)90014-I
    IITA Subjects
    Plant Breeding; Soybean; Grain Legumes
    Agrovoc Terms
    Soybeans; Breeding; Leaves; Petioles; Potatoes; Inoculation; Shoots
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Collections
    • Journal and Journal Articles5075
    copyright © 2019  IITASpace. All rights reserved.
    IITA | Open Access Repository