Show simple item record

dc.contributor.authorAkem, C.
dc.contributor.authorDashiell, Kenton E.
dc.description.abstractShoot-tips 15cm long from 15 soybean cultivars and breeding lines were individually immersed in Hoagland's solution in 1 × 14 cm test tubes, and supported by cotton plugs. All leaves were removed leaving about 1 cm of each petiole on the shoot. A 4 mm mycelial plug of Sclerotium rolfsii Sacc., taken from the periphery of a 3-day-old culture grown on acidified potato dextrose agar (PDA) media was placed between the stem and a petiole in the middle of the shoot. Tubes with shoots were then placed in a polyethylene enclosure in a growth room where the day and night temperatures averaged 31 ± 2°C and 24 ± 2°C, respectively. Relative humidity (r.h.) was maintained at 80–90% by lining the bottom of the enclosure with wet burlap. Lesions appeared on shoot tips 2 days after inoculation, and their length was measured 2, 3 and 4 days later. On three cultivars, TG × 1436-1 E, TG × 1596-2E and TG × 1614-1E, the rate of lesion expansion was significantly less than that on the other cultivars. One week after inoculation, tubes were drained, and shoots left in the chamber at 50–60% r.h. to allow sclerotia to form. Sclerotia from each shoot were collected. The cultivars TG × 1436-1 D and TG × 1596-2E produced the fewest sclerotia per shoot-tip. Sclerotial viability, determined by germination on PDA at 25 ± 2°C in darkness, ranged from 38 to 99%. This method is effective in differentiating reactions of soybean cultivars to S. rolfsii.
dc.titleDetached shoot technique to evaluate the reaction of soybean cultivars to Sclerotium rolfsii
dc.typeJournal Article
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionWest Africa
cg.authorship.typesCGIAR single centre
cg.iitasubjectPlant Breeding
cg.iitasubjectGrain Legumes
cg.accessibilitystatusLimited Access

Files in this item


There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record