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    Production of banana bunchy top virus (BBTV)-free plantain plants by in vitro culture

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    Journal Article (680.2Kb)
    Date
    2020-03-31
    Author
    Tchatchambe, N.B.J.
    Ibanda, N.
    Adheka, G.
    Onautshu, O.
    Swennen, R.
    Dhed’a, D.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    Banana Bunchy Top Disease (BBTD) caused by the Banana Bunchy Top Virus (BBTV) is one of the most important banana diseases in the Democratic Republic of Congo. This study focused on the production of BBTV-free plantain seedlings from infected banana plants. A total of 10 suckers from the French plantain Litete (Musa AAB) and the False Horn plantain Libanga Likale (Musa AAB) with advanced BBTD symptoms were collected. Meristematic apices excised from those suckers were cultured in vitro and subcultured five times. The presence of BBTV was evaluated by the Triple-Antibody Sandwich Enzyme-linked Immunosorbent Assay (TAS-ELISA). The BBTV was confirmed in all suckers prior to in vitro culture but 73.3% of Litete plantlets and 66.6% of Libanga Likale plantlets regenerated from meristematic tissues were virus-free. This indicates that in vitro culture is a simple tool to generate BBTV-free plantains.
    https://dx.doi.org/10.5897/AJAR2019.14522
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/6842
    IITA Authors ORCID
    Rony Swennenhttps://orcid.org/0000-0002-5258-9043
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.5897/AJAR2019.14522
    Research Themes
    Biotech and Plant Breeding
    IITA Subjects
    Agronomy; Banana; Food Security; Plant Diseases; Plant Production; Plantain; Tissue Culture
    Agrovoc Terms
    Banana Bunchy Top Virus; In Vitro Culture; Tissue Culture; Plantains; Bananas
    Regions
    Africa; Central Africa
    Countries
    Republic of the Congo
    Hubs
    Eastern Africa Hub
    Journals
    African Journal of Agricultural Research
    Collections
    • Journal and Journal Articles4835
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