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dc.contributor.authorTchatchambe, N.B.J.
dc.contributor.authorIbanda, N.
dc.contributor.authorAdheka, G.
dc.contributor.authorOnautshu, O.
dc.contributor.authorSwennen, R.
dc.contributor.authorDhed’a, D.
dc.date.accessioned2020-05-21T14:11:14Z
dc.date.available2020-05-21T14:11:14Z
dc.date.issued2020-03-31
dc.identifier.citationTchatchambe, N.B.J., Ibanda, N., Adheka, J.G., Onautshu, O.D., Swennen, R. & Dhed'a, D.B. (2020). Production of banana bunchy top virus (BBTV)-free plantain plants by in vitro culture. African Journal of Agricultural Research, 15(3), 361-366.
dc.identifier.issn1991-637X
dc.identifier.urihttps://hdl.handle.net/20.500.12478/6842
dc.description.abstractBanana Bunchy Top Disease (BBTD) caused by the Banana Bunchy Top Virus (BBTV) is one of the most important banana diseases in the Democratic Republic of Congo. This study focused on the production of BBTV-free plantain seedlings from infected banana plants. A total of 10 suckers from the French plantain Litete (Musa AAB) and the False Horn plantain Libanga Likale (Musa AAB) with advanced BBTD symptoms were collected. Meristematic apices excised from those suckers were cultured in vitro and subcultured five times. The presence of BBTV was evaluated by the Triple-Antibody Sandwich Enzyme-linked Immunosorbent Assay (TAS-ELISA). The BBTV was confirmed in all suckers prior to in vitro culture but 73.3% of Litete plantlets and 66.6% of Libanga Likale plantlets regenerated from meristematic tissues were virus-free. This indicates that in vitro culture is a simple tool to generate BBTV-free plantains.
dc.language.isoen
dc.subjectBanana Bunchy Top Virus
dc.subjectIn Vitro Culture
dc.subjectTissue Culture
dc.subjectPlantains
dc.subjectBananas
dc.titleProduction of banana bunchy top virus (BBTV)-free plantain plants by in vitro culture
dc.typeJournal Article
cg.contributor.crpAgriculture for Nutrition and Health
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationUniversity of Kisangani
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionCentral Africa
cg.coverage.countryRepublic of the Congo
cg.coverage.hubEastern Africa Hub
cg.researchthemeBiotech and Plant Breeding
cg.identifier.bibtexciteidTCHATCHAMBE:2020
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectAgronomy
cg.iitasubjectBanana
cg.iitasubjectFood Security
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Production
cg.iitasubjectPlantain
cg.iitasubjectTissue Culture
cg.journalAfrican Journal of Agricultural Research
cg.notesOpen Access Journal
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.5897/AJAR2019.14522
cg.iitaauthor.identifierRony Swennen: 0000-0002-5258-9043


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