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CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding

dc.contributor.authorTripathi, J.N.
dc.contributor.authorNtui, V.O.
dc.contributor.authorRon, M.
dc.contributor.authorMuiruri, S.K.
dc.contributor.authorBritt, A.
dc.contributor.authorTripathi, L.
dc.date.accessioned2020-06-04T10:10:41Z
dc.date.available2020-06-04T10:10:41Z
dc.date.issued2019
dc.identifier.citationTripathi, J.N., Ntui, V.O., Ron, M., Muiruri, S.K., Britt, A. & Tripathi, L. (2019). CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding. Communications Biology, 2(1), 1-11.
dc.identifier.issn2399-3642
dc.identifier.urihttps://hdl.handle.net/20.500.12478/6857
dc.description.abstractPresence of the integrated endogenous banana streak virus (eBSV) in the B genome of plantain (AAB) is a major challenge for breeding and dissemination of hybrids. As the eBSV activates into infectious viral particles under stress, the progenitor Musa balbisiana and its derivants, having at least one B genome, cannot be used as parents for crop improvement. Here, we report a strategy to inactivate the eBSV by editing the virus sequences. The regenerated genome-edited events of Gonja Manjaya showed mutations in the targeted sites with the potential to prevent proper transcription or/and translational into functional viral proteins. Seventy-five percent of the edited events remained asymptomatic in comparison to the non-edited control plants under water stress conditions, confirming inactivation of eBSV into infectious viral particles. This study paves the way for the improvement of B genome germplasm and its use in breeding programs to produce hybrids that can be globally disseminated.
dc.format.extent1-11
dc.language.isoen
dc.subjectBananas
dc.subjectMusa
dc.subjectDna
dc.subjectBreeding
dc.subjectViruses
dc.subjectPlantains
dc.subjectGenomes
dc.titleCRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding
dc.typeJournal Article
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationUniversity of California
cg.coverage.regionAfrica
cg.coverage.regionEast Africa
cg.coverage.countryUganda
cg.coverage.hubEastern Africa Hub
cg.researchthemeBiotech and Plant Breeding
cg.identifier.bibtexciteidTRIPATHI:2019b
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectAgronomy
cg.iitasubjectBanana
cg.iitasubjectDisease Control
cg.iitasubjectFood Security
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Health
cg.iitasubjectPlant Production
cg.iitasubjectPlantain
cg.journalCommunications Biology
cg.notesPublished online: 31 Jan 2019
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.1038/s42003-019-0288-7
cg.iitaauthor.identifierJaindra Tripathi: 0000-0002-6366-917X
cg.iitaauthor.identifierNtui: 0000-0003-4709-8087
cg.iitaauthor.identifierLeena Tripathi: 0000-0001-5723-4981


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