• Contact Us
    • Send Feedback
    • Login
    View Item 
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    •   Home
    • Journal and Journal Articles
    • Journal and Journal Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    Whole Repository
    CollectionsIssue DateRegionCountryHubAffiliationAuthorsTitlesSubject
    This Sub-collection
    Issue DateRegionCountryHubAffiliationAuthorsTitlesSubject

    My Account

    Login

    Welcome to the International Institute of Tropical Agriculture Research Repository

    What would you like to view today?

    A detached leaf assay to rapidly screen for resistance of maize to Bipolaris maydis, the causal agent of southern corn leaf blight

    Thumbnail
    View/Open
    Journal Article (1.893Mb)
    Date
    2019
    Author
    Aregbesola, E.
    Ortega-Beltran, A.
    Falade, T.
    Jonathan, G.
    Hearne, S.
    Bandyopadhyay, R.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
    Show full item record
    Abstract/Description
    Southern corn leaf blight (SCLB), caused by the fungus Bipolaris maydis, is a disease that significantly affects maize productivity across the globe. A detached leaf assay (DLA) was developed to rapidly assess maize resistance to SCLB. Several experiments were conducted to: (i) identify a highly virulent B. maydis isolate; and to determine the most appropriate (ii) phytohormone to maintain viability of maize leaf tissue, (iii) leaf age for the assay, and (iv) inoculum concentration. Once optimized, the DLA was compared with screenhouse and field experiments. Use of DLA required a maximum of 28 days for resistance assessment, in contrast to screenhouse and field tests at a minimum of 33 and 72 days, respectively. DLA positively correlated with screenhouse (r = 0.48, P = 0.08) and field experiments (r = 0.68, P = 0.008). Assessments of diverse B. maydis strains and host genotypes indicated that the DLA could be used to detect both highly virulent SCLB strains and highly resistant maize genotypes. Here we report that DLA is a rapid, reliable technique to screen maize resistance to SCLB. Use of this tool in maize breeding programs can speed up the process of identification of sources of resistance to multiple variants of SCLB.
    https://dx.doi.org/10.1007/s10658-019-01870-4
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/6886
    IITA Authors ORCID
    Alejandro Ortega-Beltranhttps://orcid.org/0000-0003-3747-8094
    Titilayo Faladehttps://orcid.org/0000-0001-5562-7861
    Ranajit Bandyopadhyayhttps://orcid.org/0000-0003-2422-4298
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.1007/s10658-019-01870-4
    Research Themes
    Plant Production and Health; Social Science and Agribusiness
    IITA Subjects
    Agronomy; Maize; Plant Breeding; Plant Diseases; Plant Health; Plant Production
    Agrovoc Terms
    Diseases; Disease Resistance; Screening; Methods; Maize; Breeding; Cochliobolus Heterostrophus; Bipolaris Maydis
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Hubs
    Headquarters and Western Africa Hub
    Journals
    European Journal of Plant Pathology
    Collections
    • Journal and Journal Articles4835
    copyright © 2019  IITASpace. All rights reserved.
    IITA | Open Access Repository