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A detached leaf assay to rapidly screen for resistance of maize to Bipolaris maydis, the causal agent of southern corn leaf blight

dc.contributor.authorAregbesola, E.
dc.contributor.authorOrtega-Beltran, A.
dc.contributor.authorFalade, T.
dc.contributor.authorJonathan, G.
dc.contributor.authorHearne, S.
dc.contributor.authorBandyopadhyay, R.
dc.date.accessioned2020-07-06T08:26:32Z
dc.date.available2020-07-06T08:26:32Z
dc.date.issued2019
dc.identifier.citationAregbesola, E., Ortega-Beltran, A., Falade, T., Jonathan, G., Hearne, S. & Bandyopadhyay, R. (2019). A detached leaf assay to rapidly screen for resistance of maize to Bipolaris maydis, the causal agent of southern corn leaf blight. European Journal of Plant Pathology, 1-13.
dc.identifier.issn0929-1873
dc.identifier.urihttps://hdl.handle.net/20.500.12478/6886
dc.description.abstractSouthern corn leaf blight (SCLB), caused by the fungus Bipolaris maydis, is a disease that significantly affects maize productivity across the globe. A detached leaf assay (DLA) was developed to rapidly assess maize resistance to SCLB. Several experiments were conducted to: (i) identify a highly virulent B. maydis isolate; and to determine the most appropriate (ii) phytohormone to maintain viability of maize leaf tissue, (iii) leaf age for the assay, and (iv) inoculum concentration. Once optimized, the DLA was compared with screenhouse and field experiments. Use of DLA required a maximum of 28 days for resistance assessment, in contrast to screenhouse and field tests at a minimum of 33 and 72 days, respectively. DLA positively correlated with screenhouse (r = 0.48, P = 0.08) and field experiments (r = 0.68, P = 0.008). Assessments of diverse B. maydis strains and host genotypes indicated that the DLA could be used to detect both highly virulent SCLB strains and highly resistant maize genotypes. Here we report that DLA is a rapid, reliable technique to screen maize resistance to SCLB. Use of this tool in maize breeding programs can speed up the process of identification of sources of resistance to multiple variants of SCLB.
dc.description.sponsorshipCGIAR Research Program on Maize
dc.description.sponsorshipBill & Melinda Gates Foundation
dc.format.extent1-13
dc.language.isoen
dc.subjectDiseases
dc.subjectDisease Resistance
dc.subjectScreening
dc.subjectMethods
dc.subjectMaize
dc.subjectBreeding
dc.subjectCochliobolus Heterostrophus
dc.subjectBipolaris Maydis
dc.titleA detached leaf assay to rapidly screen for resistance of maize to Bipolaris maydis, the causal agent of southern corn leaf blight
dc.typeJournal Article
cg.contributor.crpAgriculture for Nutrition and Health
cg.contributor.crpMaize
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationUniversity of Ibadan
cg.contributor.affiliationInternational Maize and Wheat Improvement Center
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.coverage.hubHeadquarters and Western Africa Hub
cg.researchthemePlant Production and Health
cg.researchthemeSocial Science and Agribusiness
cg.identifier.bibtexciteidAREGBESOLA:2019a
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectAgronomy
cg.iitasubjectMaize
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Health
cg.iitasubjectPlant Production
cg.journalEuropean Journal of Plant Pathology
cg.notesOpen Access Article; Published online: 26 Nov 2019
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.1007/s10658-019-01870-4
cg.iitaauthor.identifierAlejandro Ortega-Beltran: 0000-0003-3747-8094
cg.iitaauthor.identifierTitilayo Falade: 0000-0001-5562-7861
cg.iitaauthor.identifierRanajit Bandyopadhyay: 0000-0003-2422-4298


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