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    Chromosome painting facilitates anchoring reference genome sequence to chromosomes in situ and integrated karyotyping in banana (Musa spp.)

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    Journal Article (2.392Mb)
    Date
    2019
    Author
    Šimoníková, D.
    Němečková, A.
    Karafiátová, M.
    Uwimana, B.
    Swennen, R.
    Doležel, J.
    Hřibová, E.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    Oligo painting FISH was established to identify all chromosomes in banana (Musa spp.) and to anchor pseudomolecules of reference genome sequence of Musa acuminata spp. malaccensis “DH Pahang” to individual chromosomes in situ. A total of 19 chromosome/chromosome-arm specific oligo painting probes were developed and were shown to be suitable for molecular cytogenetic studies in genus Musa. For the first time, molecular karyotypes of diploid M. acuminata spp. malaccensis (A genome), M. balbisiana (B genome), and M. schizocarpa (S genome) from the Eumusa section of Musa, which contributed to the evolution of edible banana cultivars, were established. This was achieved after a combined use of oligo painting probes and a set of previously developed banana cytogenetic markers. The density of oligo painting probes was sufficient to study chromosomal rearrangements on mitotic as well as on meiotic pachytene chromosomes. This advance will enable comparative FISH mapping and identification of chromosomal translocations which accompanied genome evolution and speciation in the family Musaceae.
    https://dx.doi.org/10.3389/fpls.2019.01503
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/6909
    IITA Authors ORCID
    Brigitte Uwimanahttps://orcid.org/0000-0001-7460-9001
    Rony Swennenhttps://orcid.org/0000-0002-5258-9043
    Jaroslav Dolezelhttps://orcid.org/0000-0002-6263-0492
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.3389/fpls.2019.01503
    Research Themes
    Biotech and Plant Breeding
    IITA Subjects
    Agronomy; Banana; Genetic Improvement; Plant Breeding; Plant Genetic Resources; Plant Health; Plant Production
    Agrovoc Terms
    Bananas; Chromosomes; Karyotypes; Musa; Dna; Genomes; Genetic Structures
    Regions
    Africa; East Africa; Europe
    Countries
    Belgium; Uganda
    Hubs
    Eastern Africa Hub
    Journals
    Frontiers in Plant Science
    Collections
    • Journal and Journal Articles4463
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