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Chromosome painting facilitates anchoring reference genome sequence to chromosomes in situ and integrated karyotyping in banana (Musa spp.)

dc.contributor.authorŠimoníková, D.
dc.contributor.authorNěmečková, A.
dc.contributor.authorKarafiátová, M.
dc.contributor.authorUwimana, B.
dc.contributor.authorSwennen, R.
dc.contributor.authorDoležel, J.
dc.contributor.authorHřibová, E.
dc.date.accessioned2020-08-05T11:16:06Z
dc.date.available2020-08-05T11:16:06Z
dc.date.issued2019
dc.identifier.citationŠimoníková, D., Němečková, A., Karafiátová, M., Uwimana, B., Swennen, R., Doležel, J. & Hřibová, E. (2019). Chromosome painting facilitates anchoring reference genome sequence to chromosomes in situ and integrated karyotyping in banana (Musa spp.). Frontiers in Plant Science, 10, 1-12.
dc.identifier.issn1664-302X
dc.identifier.urihttps://hdl.handle.net/20.500.12478/6909
dc.description.abstractOligo painting FISH was established to identify all chromosomes in banana (Musa spp.) and to anchor pseudomolecules of reference genome sequence of Musa acuminata spp. malaccensis “DH Pahang” to individual chromosomes in situ. A total of 19 chromosome/chromosome-arm specific oligo painting probes were developed and were shown to be suitable for molecular cytogenetic studies in genus Musa. For the first time, molecular karyotypes of diploid M. acuminata spp. malaccensis (A genome), M. balbisiana (B genome), and M. schizocarpa (S genome) from the Eumusa section of Musa, which contributed to the evolution of edible banana cultivars, were established. This was achieved after a combined use of oligo painting probes and a set of previously developed banana cytogenetic markers. The density of oligo painting probes was sufficient to study chromosomal rearrangements on mitotic as well as on meiotic pachytene chromosomes. This advance will enable comparative FISH mapping and identification of chromosomal translocations which accompanied genome evolution and speciation in the family Musaceae.
dc.format.extent1-12
dc.language.isoen
dc.subjectBananas
dc.subjectChromosomes
dc.subjectKaryotypes
dc.subjectMusa
dc.subjectDna
dc.subjectGenomes
dc.subjectGenetic Structures
dc.titleChromosome painting facilitates anchoring reference genome sequence to chromosomes in situ and integrated karyotyping in banana (Musa spp.)
dc.typeJournal Article
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationCzech Academy of Sciences
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationBioversity International
cg.contributor.affiliationKatholieke Universiteit, Leuven
cg.coverage.regionAfrica
cg.coverage.regionEast Africa
cg.coverage.regionEurope
cg.coverage.countryBelgium
cg.coverage.countryUganda
cg.coverage.hubEastern Africa Hub
cg.researchthemeBiotech and Plant Breeding
cg.identifier.bibtexciteidSIMONIKOVA:2019
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectAgronomy
cg.iitasubjectBanana
cg.iitasubjectGenetic Improvement
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Genetic Resources
cg.iitasubjectPlant Health
cg.iitasubjectPlant Production
cg.journalFrontiers in Plant Science
cg.notesOpen Access Journal; Published online: 20 Nov 2019
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.3389/fpls.2019.01503
cg.iitaauthor.identifierBrigitte Uwimana: 0000-0001-7460-9001
cg.iitaauthor.identifierRony Swennen: 0000-0002-5258-9043
cg.iitaauthor.identifierJaroslav Dolezel: 0000-0002-6263-0492


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