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    Identification of additional/novel QTL associated with resistance to cassava green mite in a biparental mapping population

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    Journal Article (1.203Mb)
    Date
    2020-04-02
    Author
    Ezenwaka, L.
    Rabbi, I.Y.
    Onyeka, J.
    Kulakow, P.
    Egesi, C.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    Cassava green mite [CGM, Mononychellus tanajoa (Bondar)] is the most destructive dry-season pest in most cassava production areas. The pest is responsible for cassava fresh root yield losses of over 80%. Deployment of CGM resistant cultivars is the most cost-effective and sustainable approach of alleviating such production losses. The purposes of this study were to validate the stability of CGM resistance genes found in previously published results, to identify new genes for CGM resistance in bi-parental mapping population and estimate the heritability of the trait. A total of 109 F1 progeny derived from a cross between CGM resistant parent, TMEB778 and a very susceptible parent, TMEB419 were evaluated under CGM hotspot areas in Nigeria for two cropping seasons. A total of 42,204 SNP markers with MAF ≥ 0.05 were used for single-marker analysis. The most significant QTL (S12_7962234) was identified on the left arm on chromosome 12 which explained high phenotypic variance and harboured significant single nucleotide polymorphism (SNP) markers conferring resistance to CGM and leaf pubescence (LP). Colocalization of the most significant SNP associated with resistance to CGM and LP on chromosome 12 is possibly an indication of a beneficial pleiotropic effect or are physically linked. These significant SNPs markers were intersected with the gene annotations and 33 unique genes were identified within SNPs at 4 – 8MB on chromosome 12. Among these genes, nine novel candidate genes namely; Manes.12077600, Manes.12G086200, Manes.12G061200, Manes.12G083100, Manes.12G082000, Manes.12G094100, Manes.12G075600, Manes.12G091400 and Manes.12G069300 highly expressed direct link to cassava green mite resistance. Pyramiding the new QTL/genes identified on chromosome 12 in this study with previously discovered loci, such on chromosome 8, will facilitate breeding varieties that are highly resistant CGM.
    https://dx.doi.org/10.1371/journal.pone.0231008
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/6932
    IITA Authors ORCID
    Ismail Rabbihttps://orcid.org/0000-0001-9966-2941
    Peter Kulakowhttps://orcid.org/0000-0002-7574-2645
    Chiedozie Egesihttps://orcid.org/0000-0002-9063-2727
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.1371/journal.pone.0231008
    Research Themes
    Biotech and Plant Breeding
    IITA Subjects
    Agronomy; Cassava; Plant Breeding
    Agrovoc Terms
    Cassava; Roots; Yield Losses; Seeds; Loam Soils; Sandy Soils; Quantitative Trait Loci; Mites
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Hubs
    Headquarters and Western Africa Hub
    Journals
    Plos One
    Collections
    • Journal and Journal Articles5002
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