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Identification of additional/novel QTL associated with resistance to cassava green mite in a biparental mapping population

dc.contributor.authorEzenwaka, L.
dc.contributor.authorRabbi, I.Y.
dc.contributor.authorOnyeka, J.
dc.contributor.authorKulakow, P.
dc.contributor.authorEgesi, C.
dc.date.accessioned2020-08-13T14:48:32Z
dc.date.available2020-08-13T14:48:32Z
dc.date.issued2020-04-02
dc.identifier.citationEzenwaka, L., Rabbi, I., Onyeka, J., Kulakow, P. & Egesi, C. (2020). Identification of additional/novel QTL associated with resistance to cassava green mite in a biparental mapping population. Plos One, 15(4): e0231008, 1-15.
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/20.500.12478/6932
dc.description.abstractCassava green mite [CGM, Mononychellus tanajoa (Bondar)] is the most destructive dry-season pest in most cassava production areas. The pest is responsible for cassava fresh root yield losses of over 80%. Deployment of CGM resistant cultivars is the most cost-effective and sustainable approach of alleviating such production losses. The purposes of this study were to validate the stability of CGM resistance genes found in previously published results, to identify new genes for CGM resistance in bi-parental mapping population and estimate the heritability of the trait. A total of 109 F1 progeny derived from a cross between CGM resistant parent, TMEB778 and a very susceptible parent, TMEB419 were evaluated under CGM hotspot areas in Nigeria for two cropping seasons. A total of 42,204 SNP markers with MAF ≥ 0.05 were used for single-marker analysis. The most significant QTL (S12_7962234) was identified on the left arm on chromosome 12 which explained high phenotypic variance and harboured significant single nucleotide polymorphism (SNP) markers conferring resistance to CGM and leaf pubescence (LP). Colocalization of the most significant SNP associated with resistance to CGM and LP on chromosome 12 is possibly an indication of a beneficial pleiotropic effect or are physically linked. These significant SNPs markers were intersected with the gene annotations and 33 unique genes were identified within SNPs at 4 – 8MB on chromosome 12. Among these genes, nine novel candidate genes namely; Manes.12077600, Manes.12G086200, Manes.12G061200, Manes.12G083100, Manes.12G082000, Manes.12G094100, Manes.12G075600, Manes.12G091400 and Manes.12G069300 highly expressed direct link to cassava green mite resistance. Pyramiding the new QTL/genes identified on chromosome 12 in this study with previously discovered loci, such on chromosome 8, will facilitate breeding varieties that are highly resistant CGM.
dc.description.sponsorshipBill & Melinda Gates Foundation
dc.format.extent15 p.
dc.language.isoen
dc.subjectCassava
dc.subjectRoots
dc.subjectYield Losses
dc.subjectSeeds
dc.subjectLoam Soils
dc.subjectSandy Soils
dc.subjectQuantitative Trait Loci
dc.subjectMites
dc.titleIdentification of additional/novel QTL associated with resistance to cassava green mite in a biparental mapping population
dc.typeJournal Article
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationNational Root Crops Research Institute, Nigeria
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.coverage.hubHeadquarters and Western Africa Hub
cg.researchthemeBiotech and Plant Breeding
cg.identifier.bibtexciteidEZENWAKA:2020
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectAgronomy
cg.iitasubjectCassava
cg.iitasubjectPlant Breeding
cg.journalPlos One
cg.notesOpen Access Journal
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.1371/journal.pone.0231008
cg.iitaauthor.identifierIsmail Rabbi: 0000-0001-9966-2941
cg.iitaauthor.identifierPeter Kulakow: 0000-0002-7574-2645
cg.iitaauthor.identifierChiedozie Egesi: 0000-0002-9063-2727


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