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    Proteo-trancriptomic analyses reveal a large expansion of metalloprotease-like proteins in atypical venom vesicles of the wasp Meteorus pulchricornis (Braconidae)

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    Journal Article (1.581Mb)
    Date
    2021-07-19
    Author
    Gatti, J.L.
    Belghazi, M.
    Legeai, F.
    Ravallec, M.
    Frayssinet, M.
    Robin, S.
    Aboubakar-Souna, D.
    Srinivasan, R.
    Tamὸ, M.
    Poirie, M.
    Volkoff, A.N.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    Meteorus pulchricornis (Ichneumonoidea, Braconidae) is an endoparasitoid wasp of lepidopteran caterpillars. Its parasitic success relies on vesicles (named M. pulchricornis Virus-Like Particles or MpVLPs) that are synthesized in the venom gland and injected into the parasitoid host along with the venom during oviposition. In order to define the content and understand the biogenesis of these atypical vesicles, we performed a transcriptome analysis of the venom gland and a proteomic analysis of the venom and purified MpVLPs. About half of the MpVLPs and soluble venom proteins identified were unknown and no similarity with any known viral sequence was found. However, MpVLPs contained a large number of proteins labelled as metalloproteinases while the most abundant protein family in the soluble venom was that of proteins containing the Domain of Unknown Function DUF-4803. The high number of these proteins identified suggests that a large expansion of these two protein families occurred in M. pulchricornis. Therefore, although the exact mechanism of MpVLPs formation remains to be elucidated, these vesicles appear to be “metalloproteinase bombs” that may have several physiological roles in the host including modifying the functions of its immune cells. The role of DUF4803 proteins, also present in the venom of other braconids, remains to be clarified.
    https://dx.doi.org/10.3390/toxins13070502
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/7236
    IITA Authors ORCID
    Manuele Tamòhttps://orcid.org/0000-0002-5863-7421
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.3390/toxins13070502
    Research Themes
    Plant Production and Health
    Agrovoc Terms
    Meteorus; Parasitoids; Vespidae; Braconidae; Viruses; Proteomics; Venom apparatus
    Hubs
    Headquarters and Western Africa Hub
    Journals
    Toxins
    Collections
    • Journal and Journal Articles4835
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