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dc.contributor.authorGatti, J.L.
dc.contributor.authorBelghazi, M.
dc.contributor.authorLegeai, F.
dc.contributor.authorRavallec, M.
dc.contributor.authorFrayssinet, M.
dc.contributor.authorRobin, S.
dc.contributor.authorAboubakar-Souna, D.
dc.contributor.authorSrinivasan, R.
dc.contributor.authorTamὸ, M.
dc.contributor.authorPoirie, M.
dc.contributor.authorVolkoff, A.N.
dc.date.accessioned2021-08-27T15:23:39Z
dc.date.available2021-08-27T15:23:39Z
dc.date.issued2021-07-19
dc.identifier.citationGatti, J.L., Belghazi, M., Legeai, F., Ravallec, M., Frayssinet, M., Robin, S., ... & Volkoff, A.N. (2021). Proteo-trancriptomic analyses reveal a large expansion of metalloprotease-like proteins in atypical venom vesicles of the wasp Meteorus pulchricornis (Braconidae). Toxins, 13(7): 502, 1-36.
dc.identifier.issn2072-6651
dc.identifier.urihttps://hdl.handle.net/20.500.12478/7236
dc.description.abstractMeteorus pulchricornis (Ichneumonoidea, Braconidae) is an endoparasitoid wasp of lepidopteran caterpillars. Its parasitic success relies on vesicles (named M. pulchricornis Virus-Like Particles or MpVLPs) that are synthesized in the venom gland and injected into the parasitoid host along with the venom during oviposition. In order to define the content and understand the biogenesis of these atypical vesicles, we performed a transcriptome analysis of the venom gland and a proteomic analysis of the venom and purified MpVLPs. About half of the MpVLPs and soluble venom proteins identified were unknown and no similarity with any known viral sequence was found. However, MpVLPs contained a large number of proteins labelled as metalloproteinases while the most abundant protein family in the soluble venom was that of proteins containing the Domain of Unknown Function DUF-4803. The high number of these proteins identified suggests that a large expansion of these two protein families occurred in M. pulchricornis. Therefore, although the exact mechanism of MpVLPs formation remains to be elucidated, these vesicles appear to be “metalloproteinase bombs” that may have several physiological roles in the host including modifying the functions of its immune cells. The role of DUF4803 proteins, also present in the venom of other braconids, remains to be clarified.
dc.description.sponsorshipFrench National Agency of Research
dc.description.sponsorshipDepartment of Plant Health
dc.description.sponsorshipFrench National Institute for Research in Agriculture, Food and Environment
dc.description.sponsorshipFrench Government
dc.format.extent1-36
dc.language.isoen
dc.subjectMeteorus
dc.subjectParasitoids
dc.subjectVespidae
dc.subjectBraconidae
dc.subjectViruses
dc.subjectProteomics
dc.subjectVenom apparatus
dc.titleProteo-trancriptomic analyses reveal a large expansion of metalloprotease-like proteins in atypical venom vesicles of the wasp Meteorus pulchricornis (Braconidae)
dc.typeJournal Article
cg.contributor.crpGrain Legumes
cg.contributor.affiliationCôte d'Azur University
cg.contributor.affiliationAix-Marseille University
cg.contributor.affiliationUniversité de Rennes 1
cg.contributor.affiliationUniversité Montpellier
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationWorld Vegetable Center
cg.coverage.hubHeadquarters and Western Africa Hub
cg.researchthemePlant Production and Health
cg.identifier.bibtexciteidGATTI:2021
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.journalToxins
cg.notesOpen Access Journal
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.3390/toxins13070502
cg.iitaauthor.identifierManuele Tamò: 0000-0002-5863-7421
cg.futureupdate.requiredNo
cg.identifier.issue7: 502
cg.identifier.volume13


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