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    Cytological and molecular characterization for ploidy determination in yams (Dioscorea spp.)

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    Journal Article (2.154Mb)
    Date
    2021
    Author
    Gatarira, C.
    Sladekova, L.
    Nemeckova, A.
    Simonikova, D.
    Paliwal, R.
    Asfaw, A.
    Abberton, M.
    Gueye, B.
    Asiedu, R.
    Cizkova, J.
    Hribova, E.
    Dolezel, J.
    De Koeyer, D .
    Adetimirin, V.
    Bhattacharjee, R.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    Yam (Dioscorea spp.) is a monocotyledonous herbaceous vine plant grown in the tropics and subtropics. It is a multi-species plant with varied intra- and interspecific ploidy levels. Of the 600 species, 11 are cultivated supporting the livelihood of over 300 million people. The paucity of information on ploidy and the genomic constitution is a significant challenge to the crop’s genetic improvement through crossbreeding. The objective of this study was to investigate the ploidy levels of 236 accessions across six cultivated and two wild species using chromosome counting, flow cytometry and genotyping-based ploidy determination methods. Results obtained from chromosome counting and genotyping-based ploidy determination were in agreement. In majority of the accessions, chromosome counting and flow cytometry were congruent, allowing future rapid screening of ploidy levels using flow cytometry. Among cultivated accessions, 168 (71%) were diploid, 50 (21%) were triploid, and 12 (5%) were tetraploid. Two wild species included in the study were diploids. Resolution of ploidy level in yams offers opportunities for implementing successful breeding programmes through intra- and interspecific hybridization.
    https://dx.doi.org/10.3390/agronomy11101897
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/7262
    IITA Authors ORCID
    Asrat Asfawhttps://orcid.org/0000-0002-4859-0631
    Michael Abbertonhttps://orcid.org/0000-0003-2555-9591
    Badara GUEYEhttps://orcid.org/0000-0003-3157-8772
    Robert Asieduhttps://orcid.org/0000-0001-8943-2376
    David De Koeyerhttps://orcid.org/0000-0001-8064-6538
    Ranjana Bhattacharjeehttps://orcid.org/0000-0002-5184-5930
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.3390/agronomy11101897
    Research Themes
    Biotech and Plant Breeding
    IITA Subjects
    Agronomy; Food Security; Plant Breeding; Plant Production; Value Chains; Yam
    Agrovoc Terms
    Yams; Flow Cytometry; Chromosomes; Ploidy; West Africa
    Regions
    Africa; West Africa
    Countries
    Benin (Dahomey); Cote d’Ivoire (Ivory Coast); Gabon; Ghana; Guinea; Nigeria; Republic of the Congo; Sierra Leone; Togo
    Hubs
    Headquarters and Western Africa Hub
    Journals
    Agronomy
    Collections
    • Journal and Journal Articles4836
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