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Cytological and molecular characterization for ploidy determination in yams (Dioscorea spp.)

dc.contributor.authorGatarira, C.
dc.contributor.authorSladekova, L.
dc.contributor.authorNemeckova, A.
dc.contributor.authorSimonikova, D.
dc.contributor.authorPaliwal, R.
dc.contributor.authorAsfaw, A.
dc.contributor.authorAbberton, M.
dc.contributor.authorGueye, B.
dc.contributor.authorAsiedu, R.
dc.contributor.authorCizkova, J.
dc.contributor.authorHribova, E.
dc.contributor.authorDolezel, J.
dc.contributor.authorDe Koeyer, D .
dc.contributor.authorAdetimirin, V.
dc.contributor.authorBhattacharjee, R.
dc.date.accessioned2021-10-06T11:44:10Z
dc.date.available2021-10-06T11:44:10Z
dc.date.issued2021
dc.identifier.citationGatarira, C., Sládeková, L., Němečková, A., Šimoníková, D., Paliwal, R., Asfaw, A., ... & Bhattacharjee, R. (2021). Cytological and Molecular Characterization for Ploidy Determination in Yams (Dioscorea spp.). Agronomy, 11(10), 1897: 1-13.
dc.identifier.issn2073-4395
dc.identifier.urihttps://hdl.handle.net/20.500.12478/7262
dc.description.abstractYam (Dioscorea spp.) is a monocotyledonous herbaceous vine plant grown in the tropics and subtropics. It is a multi-species plant with varied intra- and interspecific ploidy levels. Of the 600 species, 11 are cultivated supporting the livelihood of over 300 million people. The paucity of information on ploidy and the genomic constitution is a significant challenge to the crop’s genetic improvement through crossbreeding. The objective of this study was to investigate the ploidy levels of 236 accessions across six cultivated and two wild species using chromosome counting, flow cytometry and genotyping-based ploidy determination methods. Results obtained from chromosome counting and genotyping-based ploidy determination were in agreement. In majority of the accessions, chromosome counting and flow cytometry were congruent, allowing future rapid screening of ploidy levels using flow cytometry. Among cultivated accessions, 168 (71%) were diploid, 50 (21%) were triploid, and 12 (5%) were tetraploid. Two wild species included in the study were diploids. Resolution of ploidy level in yams offers opportunities for implementing successful breeding programmes through intra- and interspecific hybridization.
dc.description.sponsorshipBill & Melinda Gates Foundation
dc.format.extent1-13
dc.language.isoen
dc.subjectYams
dc.subjectFlow Cytometry
dc.subjectChromosomes
dc.subjectPloidy
dc.subjectWest Africa
dc.titleCytological and molecular characterization for ploidy determination in yams (Dioscorea spp.)
dc.typeJournal Article
cg.contributor.crpGenebanks
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationUniversity of Ibadan
cg.contributor.affiliationInstitute of Experimental Botany of the Czech Academy of Sciences
cg.contributor.affiliationAgriculture and Agri-Food Canada
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryBenin (Dahomey)
cg.coverage.countryCote d’Ivoire (Ivory Coast)
cg.coverage.countryGabon
cg.coverage.countryGhana
cg.coverage.countryGuinea
cg.coverage.countryNigeria
cg.coverage.countryRepublic of the Congo
cg.coverage.countrySierra Leone
cg.coverage.countryTogo
cg.coverage.hubHeadquarters and Western Africa Hub
cg.researchthemeBiotech and Plant Breeding
cg.identifier.bibtexciteidGATARIRA:2021
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectAgronomy
cg.iitasubjectFood Security
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Production
cg.iitasubjectValue Chains
cg.iitasubjectYam
cg.journalAgronomy
cg.notesOpen Access Journal; Published online: 22 Sep 2021
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.3390/agronomy11101897
cg.iitaauthor.identifierAsrat Asfaw: 0000-0002-4859-0631
cg.iitaauthor.identifierMichael Abberton: 0000-0003-2555-9591
cg.iitaauthor.identifierBadara GUEYE: 0000-0003-3157-8772
cg.iitaauthor.identifierRobert Asiedu: 0000-0001-8943-2376
cg.iitaauthor.identifierDavid De Koeyer: 0000-0001-8064-6538
cg.iitaauthor.identifierRanjana Bhattacharjee: 0000-0002-5184-5930
cg.futureupdate.requiredNo
cg.identifier.issue10: 1897
cg.identifier.volume11


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