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    Comparative assessment of effectiveness of alternative genotyping assays for characterizing carotenoids accumulation in tropical maize inbred lines

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    Journal Article (1.015Mb)
    Date
    2021
    Author
    Sayadi Maazou, A.R.
    Gedil, M.
    Adetimirin, V.
    Meseka, S.K.
    Mengesha Abera, W.
    Babalola, D.
    Offornedo, Q.N.
    Menkir, A.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    The development of maize varieties with increased concentration of Provitamin A (PVA) is an effective and affordable strategy to combat vitamin A deficiency in developing nations. However, the considerably high cost of carotene analysis poses a major challenge for maize PVA biofortification, prompting the use of marker-assisted selection. Presently, two types of genotyping with PVA trait-linked functional markers have been developed and extensively used in breeding programs. The two systems are low throughput gel-based genotyping and genotyping with Kompetitive Allele-Specific PCR (KASP) single nucleotide polymorphism (SNPs) markers. Although the KASP SNPs genotyping was developed to replace the gel-based genotyping, studies have not been conducted to compare the effectiveness of the KASP SNPs markers with the gel-based markers. This study was conducted to assess the carotenoid content of 64 tropical PVA biofortified maize inbred lines containing temperate germplasm in their genetic backgrounds and screen them with both gel-based and KASP markers of PSY1, LCYE and crtRB1 genes. Many of the 64 inbred lines had PVA concentrations surpassing the 15 µg/g provitamin A breeding target set by the HarvestPlus Challenge Program. Favorable alleles of crtRB1, crtRB1 and the KASP SNPs markers were detected in 25 inbred lines with high PVA concentrations. Inbred lines with the favorable alleles of LCYE had the highest concentrations of non-PVA carotenoids, whereas those with the favorable alleles of crtRB1 had high levels of PVA carotenoids. Data from the sequenced region of LCYE revealed one SNP in the first intron that clearly differentiated the high and low β-carotene maize inbred lines. The results of our study demonstrate that the automated KASP SNPs markers can replace the gel-based genotyping for screening a large number of early generation maize inbred lines for PVA content.
    https://dx.doi.org/10.3390/agronomy11102022
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/7315
    IITA Authors ORCID
    Melaku Gedilhttps://orcid.org/0000-0002-6258-6014
    SILVESTRO MESEKAhttps://orcid.org/0000-0003-1004-2450
    Wende Mengeshahttps://orcid.org/0000-0002-2239-7323
    Abebe Menkirhttps://orcid.org/0000-0002-5907-9177
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.3390/agronomy11102022
    Research Themes
    Biotech and Plant Breeding
    IITA Subjects
    Agronomy; Biofortification; Food Security; Maize; Plant Breeding; Plant Production
    Agrovoc Terms
    Provitamins; Carotenoids; Biofortification; Marker Assisted Selection; Maize; Inbred Lines; Nigeria
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Hubs
    Headquarters and Western Africa Hub
    Journals
    Agronomy
    Collections
    • Journal and Journal Articles4835
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