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Comparative assessment of effectiveness of alternative genotyping assays for characterizing carotenoids accumulation in tropical maize inbred lines

dc.contributor.authorSayadi Maazou, A.R.
dc.contributor.authorGedil, M.
dc.contributor.authorAdetimirin, V.
dc.contributor.authorMeseka, S.K.
dc.contributor.authorMengesha Abera, W.
dc.contributor.authorBabalola, D.
dc.contributor.authorOffornedo, Q.N.
dc.contributor.authorMenkir, A.
dc.date.accessioned2022-01-24T08:44:30Z
dc.date.available2022-01-24T08:44:30Z
dc.date.issued2021
dc.identifier.citationSayadi Maazou, A.R., Gedil, M., Adetimirin, V., Meseka, S.K., Mengesha Abera, W., Babalola, D., ... & Menkir, A. (2021). Comparative assessment of effectiveness of alternative genotyping assays for characterizing carotenoids accumulation in tropical maize inbred lines. Agronomy, 11(10), 2022: 1-17.
dc.identifier.issn2073-4395
dc.identifier.urihttps://hdl.handle.net/20.500.12478/7315
dc.description.abstractThe development of maize varieties with increased concentration of Provitamin A (PVA) is an effective and affordable strategy to combat vitamin A deficiency in developing nations. However, the considerably high cost of carotene analysis poses a major challenge for maize PVA biofortification, prompting the use of marker-assisted selection. Presently, two types of genotyping with PVA trait-linked functional markers have been developed and extensively used in breeding programs. The two systems are low throughput gel-based genotyping and genotyping with Kompetitive Allele-Specific PCR (KASP) single nucleotide polymorphism (SNPs) markers. Although the KASP SNPs genotyping was developed to replace the gel-based genotyping, studies have not been conducted to compare the effectiveness of the KASP SNPs markers with the gel-based markers. This study was conducted to assess the carotenoid content of 64 tropical PVA biofortified maize inbred lines containing temperate germplasm in their genetic backgrounds and screen them with both gel-based and KASP markers of PSY1, LCYE and crtRB1 genes. Many of the 64 inbred lines had PVA concentrations surpassing the 15 µg/g provitamin A breeding target set by the HarvestPlus Challenge Program. Favorable alleles of crtRB1, crtRB1 and the KASP SNPs markers were detected in 25 inbred lines with high PVA concentrations. Inbred lines with the favorable alleles of LCYE had the highest concentrations of non-PVA carotenoids, whereas those with the favorable alleles of crtRB1 had high levels of PVA carotenoids. Data from the sequenced region of LCYE revealed one SNP in the first intron that clearly differentiated the high and low β-carotene maize inbred lines. The results of our study demonstrate that the automated KASP SNPs markers can replace the gel-based genotyping for screening a large number of early generation maize inbred lines for PVA content.
dc.description.sponsorshipAfrican Union
dc.description.sponsorshipBill & Melinda Gates Foundation
dc.format.extent1-17
dc.language.isoen
dc.subjectProvitamins
dc.subjectCarotenoids
dc.subjectBiofortification
dc.subjectMarker Assisted Selection
dc.subjectMaize
dc.subjectInbred Lines
dc.subjectNigeria
dc.titleComparative assessment of effectiveness of alternative genotyping assays for characterizing carotenoids accumulation in tropical maize inbred lines
dc.typeJournal Article
cg.contributor.crpMaize
cg.contributor.affiliationUniversity of Ibadan
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.coverage.hubHeadquarters and Western Africa Hub
cg.researchthemeBiotech and Plant Breeding
cg.identifier.bibtexciteidSAYADIMAAZOU:2021
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectAgronomy
cg.iitasubjectBiofortification
cg.iitasubjectFood Security
cg.iitasubjectMaize
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Production
cg.journalAgronomy
cg.notesOpen access Journal; Published online: 09 Oct 2021
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.3390/agronomy11102022
cg.iitaauthor.identifierMelaku Gedil: 0000-0002-6258-6014
cg.iitaauthor.identifierSILVESTRO MESEKA: 0000-0003-1004-2450
cg.iitaauthor.identifierWende Mengesha: 0000-0002-2239-7323
cg.iitaauthor.identifierAbebe Menkir: 0000-0002-5907-9177
cg.futureupdate.requiredNo
cg.identifier.issue10: 2022
cg.identifier.volume11


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