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    Drying banana seeds for ex situ conservation

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    Journal Article (1.255Mb)
    Date
    2022-01-12
    Author
    Kallow, S.
    Zuluaga, M.G.
    Sleziak, N.
    Nugraha, B.
    Mertens, A.
    Janssens, S.
    Gueco, L.
    Valle-Descalsota, M.L.
    Vu, T.D.
    Vu, D.T.
    Li, L.T.
    Vandelook, F.
    Dickie, J.
    Verboven, P.
    Swennen, R.
    Panis, B.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    The ability of seeds to withstand drying is fundamental to ex situ seed conservation but drying responses are not well knownfor most wild species including crop wild relatives. We look at drying responses of seeds of Musa acuminata and Musa balbisiana, the two primary wild relatives of bananas and plantains, using the following four experimental approaches: (i) We equilibrated seeds to a range of relative humidity (RH) levels using non-saturated lithium chloride solutions and subsequently measured moisture content (MC) and viability. At each humidity level we tested viability using embryo rescue (ER), tetrazolium chloride staining and germination in an incubator. We found that seed viability was not reduced when seeds were dried to 4% equilibrium relative humidity (eRH; equating to 2.5% MC). (ii) We assessed viability of mature and less mature seeds using ER and germination in the soil and tested responses to drying. Findings showed that seeds must be fully mature to germinate and immature seeds had negligible viability. (iii) We dried seeds extracted from ripe/unripe fruit to 35–40% eRH at different rates and tested viability with germination tests in the soil. Seeds from unripe fruit lost viability when dried and especially when dried faster; seeds from ripe fruit only lost viability when fast dried. (iv) Finally, we dried and re-imbibed mature and less mature seeds and measured embryo shrinkage and volume change using X-ray computer tomography. Embryos of less mature seeds shrank significantly when dried to 15% eRH from 0.468 to 0.262 mm3, but embryos of mature seeds did not. Based on our results, mature seeds from ripe fruit are desiccation tolerant to moisture levels required for seed genebanking but embryos from immature seeds are mechanistically less able to withstand desiccation, especially when water potential gradients are high.
    https://doi.org/10.1093/conphys/coab099
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/7376
    IITA Authors ORCID
    Rony Swennenhttps://orcid.org/0000-0002-5258-9043
    Digital Object Identifier (DOI)
    https://doi.org/10.1093/conphys/coab099
    Research Themes
    Social Science and Agribusiness
    IITA Subjects
    Banana; Genetic Improvement; Plant Genetic Resources
    Agrovoc Terms
    Crop Wild Relatives; Tolerance; Genetic Resources; Genebanks; Bananas
    Regions
    ACP; Asia; Europe; Southeast Asia
    Countries
    Belgium; Philippines; Vietnam
    Hubs
    Eastern Africa Hub
    Journals
    Conservation Physiology
    Collections
    • Journal and Journal Articles4835
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