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    Mediation of potato-potato cyst nematode, G. rostochiensis interaction by specific root exudate compounds

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    Journal Article (1.642Mb)
    Date
    2020
    Author
    Ochola, J.
    Cortada-Gonzalez, L.
    Ng’ang’a, M.
    Hassanali, A.
    Coyne, D.
    Torto, B.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    Potato (Solanum tuberosum) is a widely consumed staple food crop worldwide whose production is threatened by potato cyst nematodes (PCN). To infect a host, PCN eggs first need to be stimulated to hatch by chemical components in the host root exudates, yet it remains unknown how most root exudate components influence PCN behavior. Here, we evaluated the influence of eight compounds identified by LC-QqQ-MS in the root exudate of potato on the hatching response of the PCN, Globodera rostochiensis at varying doses. The eight compounds included the amino acids tyrosine, tryptophan and phenylalanine; phytohormones zeatin and methyl dihydrojasmonate; steroidal glycoalkaloids α-solanine and α-chaconine and the steroidal alkaloid solanidine. We additionally tested two other Solanaceae steroidal alkaloids, solasodine and tomatidine, previously identified in the root exudates of tomato, an alternative host for PCN. In dose-response assays with the individual compounds, the known PCN hatching factors α-chaconine and α-solanine stimulated the highest number of eggs to hatch, ∼47 and ∼42%, respectively, whereas the steroidal alkaloids (aglycones), solanidine and solasodine and potato root exudate (PRE) were intermediate, 28% each and 21%, respectively, with tomatidine eliciting the lowest hatching response 13%. However, ∼60% of the hatched juveniles failed to emerge from the cyst, which was compound- and concentration-dependent. The amino acids, phytohormones and the negative control (1% DMSO in water), however, were generally non-stimulatory. The use of steroidal glycoalkaloids and their aglycones in the suicidal hatching of PCN offers promise as an environmentally sustainable approach to manage this pest.
    https://dx.doi.org/10.3389/fpls.2020.00649
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/7414
    IITA Authors ORCID
    LAURA CORTADAhttps://orcid.org/0000-0002-5953-3798
    Daniel Coynehttps://orcid.org/0000-0002-2030-6328
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.3389/fpls.2020.00649
    Research Themes
    Biotech and Plant Breeding; Natural Resource Management
    IITA Subjects
    Agronomy; Disease Control; Food Security; Plant Breeding; Plant Diseases; Plant Health; Plant Production
    Agrovoc Terms
    Potatoes; Globodera; Hatcheries; Semiochemicals; Steroids; Glycoalkaloids
    Regions
    Africa; East Africa
    Countries
    Kenya
    Hubs
    Eastern Africa Hub
    Journals
    Frontiers in Plant Science
    Collections
    • Journal and Journal Articles4835
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