| dc.contributor.author | Ochola, J. |
| dc.contributor.author | Cortada-Gonzalez, L. |
| dc.contributor.author | Ng’ang’a, M. |
| dc.contributor.author | Hassanali, A. |
| dc.contributor.author | Coyne, D. |
| dc.contributor.author | Torto, B. |
| dc.date.accessioned | 2022-04-13T09:44:27Z |
| dc.date.available | 2022-04-13T09:44:27Z |
| dc.date.issued | 2020 |
| dc.identifier.citation | Ochola, J., Cortada, L., Ng’ang’a, M., Hassanali, A., Coyne, D. & Torto, B. (2020). Mediation of potato-potato cyst nematode, G. rostochiensis interaction by specific root exudate compounds. Frontiers in Plant Science, 11: 649, 1-11. |
| dc.identifier.issn | 1664-462X |
| dc.identifier.uri | https://hdl.handle.net/20.500.12478/7414 |
| dc.description.abstract | Potato (Solanum tuberosum) is a widely consumed staple food crop worldwide whose production is threatened by potato cyst nematodes (PCN). To infect a host, PCN eggs first need to be stimulated to hatch by chemical components in the host root exudates, yet it remains unknown how most root exudate components influence PCN behavior. Here, we evaluated the influence of eight compounds identified by LC-QqQ-MS in the root exudate of potato on the hatching response of the PCN, Globodera rostochiensis at varying doses. The eight compounds included the amino acids tyrosine, tryptophan and phenylalanine; phytohormones zeatin and methyl dihydrojasmonate; steroidal glycoalkaloids α-solanine and α-chaconine and the steroidal alkaloid solanidine. We additionally tested two other Solanaceae steroidal alkaloids, solasodine and tomatidine, previously identified in the root exudates of tomato, an alternative host for PCN. In dose-response assays with the individual compounds, the known PCN hatching factors α-chaconine and α-solanine stimulated the highest number of eggs to hatch, ∼47 and ∼42%, respectively, whereas the steroidal alkaloids (aglycones), solanidine and solasodine and potato root exudate (PRE) were intermediate, 28% each and 21%, respectively, with tomatidine eliciting the lowest hatching response 13%. However, ∼60% of the hatched juveniles failed to emerge from the cyst, which was compound- and concentration-dependent. The amino acids, phytohormones and the negative control (1% DMSO in water), however, were generally non-stimulatory. The use of steroidal glycoalkaloids and their aglycones in the suicidal hatching of PCN offers promise as an environmentally sustainable approach to manage this pest. |
| dc.description.sponsorship | North Carolina State University |
| dc.description.sponsorship | Bill & Melinda Gates Foundation |
| dc.description.sponsorship | Department for International Development, United Kingdom |
| dc.description.sponsorship | Swedish International Development Cooperation Agency |
| dc.description.sponsorship | Swiss Agency for Development and Cooperation |
| dc.description.sponsorship | Federal Democratic Republic of Ethiopia |
| dc.description.sponsorship | Kenyan Government |
| dc.format.extent | 1-11 |
| dc.language.iso | en |
| dc.subject | Potatoes |
| dc.subject | Globodera |
| dc.subject | Hatcheries |
| dc.subject | Semiochemicals |
| dc.subject | Steroids |
| dc.subject | Glycoalkaloids |
| dc.title | Mediation of potato-potato cyst nematode, G. rostochiensis interaction by specific root exudate compounds |
| dc.type | Journal Article |
| cg.contributor.crp | Roots, Tubers and Bananas |
| cg.contributor.affiliation | International Centre of Insect Physiology and Ecology |
| cg.contributor.affiliation | Kenyatta University |
| cg.contributor.affiliation | Ghent University |
| cg.contributor.affiliation | International Institute of Tropical Agriculture |
| cg.coverage.region | Africa |
| cg.coverage.region | East Africa |
| cg.coverage.country | Kenya |
| cg.coverage.hub | Eastern Africa Hub |
| cg.researchtheme | Biotech and Plant Breeding |
| cg.researchtheme | Natural Resource Management |
| cg.identifier.bibtexciteid | OCHOLA:2020 |
| cg.isijournal | ISI Journal |
| cg.authorship.types | CGIAR and developing country institute |
| cg.iitasubject | Agronomy |
| cg.iitasubject | Disease Control |
| cg.iitasubject | Food Security |
| cg.iitasubject | Plant Breeding |
| cg.iitasubject | Plant Diseases |
| cg.iitasubject | Plant Health |
| cg.iitasubject | Plant Production |
| cg.journal | Frontiers in Plant Science |
| cg.notes | Open Access Journal; Published online: 10 June 2020 |
| cg.accessibilitystatus | Open Access |
| cg.reviewstatus | Peer Review |
| cg.usagerightslicense | Creative Commons Attribution 4.0 (CC BY 0.0) |
| cg.targetaudience | Scientists |
| cg.identifier.doi | https://dx.doi.org/10.3389/fpls.2020.00649 |
| cg.iitaauthor.identifier | LAURA CORTADA: 0000-0002-5953-3798 |
| cg.iitaauthor.identifier | Daniel Coyne: 0000-0002-2030-6328 |
| cg.futureupdate.required | No |
| cg.identifier.issue | 649 |
| cg.identifier.volume | 11 |
