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    Paternity assignment in white guinea yam (Dioscorea Rotundata) half-sib Progenies from polycross mating design using SNP markers

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    Journal Article (1.467Mb)
    Date
    2020
    Author
    Norman, P.E.
    Agre, P.A.
    Danquah, A.
    Tongoona, P.B.
    Danquah, E.Y.
    De Koeyer, D.
    Ikeogu, U.N.
    Asiedu, R.
    Asfaw, A.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    White Guinea yam is mostly a dioecious outcrossing crop with male and female flowers produced on distinct plants. Fertile parents produce high fruit set in an open pollination polycross block, which is a cost-effective and convenient way of generating variability in yam breeding. However, the pollen parent of progeny from polycross mating is usually unknown. This study aimed to determine paternity in white Guinea yam half-sib progenies from polycross mating design. A total of 394 half-sib progenies from random open pollination involving nine female and three male parents was genotyped with 6602 SNP markers from DArTSeq platform to recover full pedigree. A higher proportion of expected heterozygosity, allelic richness, and evenness were observed in the half-sib progenies. A complete pedigree was established for all progenies from two families (TDr1685 and TDr1688) with 100% accuracy, while in the remaining families, paternity was assigned successfully only for 56 to 98% of the progenies. Our results indicated unequal paternal contribution under natural open pollination in yam, suggesting unequal pollen migrations or gene flow among the crossing parents. A total of 3.8% of progenies lacking paternal identity due to foreign pollen contamination outside the polycross block was observed. This study established the efficient determination of parental reconstruction and allelic contributions in the white Guinea yam half-sib progenies generated from open pollination polycross using SNP markers. Findings are useful for parental reconstruction, accurate dissection of the genetic effects, and selection in white Guinea yam breeding program utilizing polycross mating design.
    https://dx.doi.org/10.3390/plants9040527
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/7438
    IITA Authors ORCID
    Paterne AGREhttps://orcid.org/0000-0003-1231-2530
    David De Koeyerhttps://orcid.org/0000-0001-8064-6538
    Ugochukwu Nathaniel Ikeoguhttps://orcid.org/0000-0001-6478-261X
    Robert Asieduhttps://orcid.org/0000-0001-8943-2376
    Asrat Asfawhttps://orcid.org/0000-0002-4859-0631
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.3390/plants9040527
    Research Themes
    Biotech and Plant Breeding
    IITA Subjects
    Agronomy; Food Security; Plant Breeding; Plant Production; Yam
    Agrovoc Terms
    Yams; Dioscorea Rotundata; Dna; Food Production; Paternity
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Hubs
    Headquarters and Western Africa Hub
    Journals
    Plants
    Collections
    • Journal and Journal Articles4835
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