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Paternity assignment in white guinea yam (Dioscorea Rotundata) half-sib Progenies from polycross mating design using SNP markers

dc.contributor.authorNorman, P.E.
dc.contributor.authorAgre, P.A.
dc.contributor.authorDanquah, A.
dc.contributor.authorTongoona, P.B.
dc.contributor.authorDanquah, E.Y.
dc.contributor.authorDe Koeyer, D.
dc.contributor.authorIkeogu, U.N.
dc.contributor.authorAsiedu, R.
dc.contributor.authorAsfaw, A.
dc.date.accessioned2022-05-04T11:53:49Z
dc.date.available2022-05-04T11:53:49Z
dc.date.issued2020
dc.identifier.citationNorman, P.E., Agre, P.A., Danquah, A., Tongoona, P.B., Danquah, E.Y., De Koeyer, D., ... & Asfaw, A. (2020). Paternity assignment in white guinea yam (Dioscorea Rotundata) half-sib Progenies from polycross mating design using SNP markers. Plants, 9(4): 527, 1-15.
dc.identifier.issn2223-7747
dc.identifier.urihttps://hdl.handle.net/20.500.12478/7438
dc.description.abstractWhite Guinea yam is mostly a dioecious outcrossing crop with male and female flowers produced on distinct plants. Fertile parents produce high fruit set in an open pollination polycross block, which is a cost-effective and convenient way of generating variability in yam breeding. However, the pollen parent of progeny from polycross mating is usually unknown. This study aimed to determine paternity in white Guinea yam half-sib progenies from polycross mating design. A total of 394 half-sib progenies from random open pollination involving nine female and three male parents was genotyped with 6602 SNP markers from DArTSeq platform to recover full pedigree. A higher proportion of expected heterozygosity, allelic richness, and evenness were observed in the half-sib progenies. A complete pedigree was established for all progenies from two families (TDr1685 and TDr1688) with 100% accuracy, while in the remaining families, paternity was assigned successfully only for 56 to 98% of the progenies. Our results indicated unequal paternal contribution under natural open pollination in yam, suggesting unequal pollen migrations or gene flow among the crossing parents. A total of 3.8% of progenies lacking paternal identity due to foreign pollen contamination outside the polycross block was observed. This study established the efficient determination of parental reconstruction and allelic contributions in the white Guinea yam half-sib progenies generated from open pollination polycross using SNP markers. Findings are useful for parental reconstruction, accurate dissection of the genetic effects, and selection in white Guinea yam breeding program utilizing polycross mating design.
dc.description.sponsorshipBill & Melinda Gates Foundation
dc.format.extent1-15
dc.language.isoen
dc.subjectYams
dc.subjectDioscorea Rotundata
dc.subjectDna
dc.subjectFood Production
dc.subjectPaternity
dc.titlePaternity assignment in white guinea yam (Dioscorea Rotundata) half-sib Progenies from polycross mating design using SNP markers
dc.typeJournal Article
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationSierra Leone Agricultural Research Institute
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationUniversity of Ghana
cg.contributor.affiliationAgriculture and Agri-Food Canada
cg.contributor.affiliationCornell University
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.coverage.hubHeadquarters and Western Africa Hub
cg.researchthemeBiotech and Plant Breeding
cg.identifier.bibtexciteidNORMAN:2020
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectAgronomy
cg.iitasubjectFood Security
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Production
cg.iitasubjectYam
cg.journalPlants
cg.notesOpen Access Journal; Published online: 19 April 2020
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.3390/plants9040527
cg.iitaauthor.identifierPaterne AGRE: 0000-0003-1231-2530
cg.iitaauthor.identifierDavid De Koeyer: 0000-0001-8064-6538
cg.iitaauthor.identifierUgochukwu Nathaniel Ikeogu: 0000-0001-6478-261X
cg.iitaauthor.identifierRobert Asiedu: 0000-0001-8943-2376
cg.iitaauthor.identifierAsrat Asfaw: 0000-0002-4859-0631
cg.futureupdate.requiredNo
cg.identifier.issue4: 527
cg.identifier.volume9


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