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    Gene expression and metabolite profiling of thirteen Nigerian cassava landraces to elucidate starch and carotenoid composition

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    Journal Article (628.9Kb)
    Date
    2020
    Author
    Olayide, P.
    Large, A.
    Stridh, L.
    Rabbi, I.
    Baldermann, S.
    Stavolone, L.
    Alexandersson, E.
    Type
    Journal Article
    Review Status
    Peer Review
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    The prevalence of vitamin A deficiency in sub-Saharan Africa necessitates effective approaches to improve provitamin A content of major staple crops. Cassava holds much promise for food security in sub-Saharan Africa, but a negative correlation between β-carotene, a provitamin A carotenoid, and dry matter content has been reported, which poses a challenge to cassava biofortification by conventional breeding. To identify suitable material for genetic transformation in tissue culture with the overall aim to increase β-carotene and maintain starch content as well as better understand carotenoid composition, root and leaf tissues from thirteen field-grown cassava landraces were analyzed for agronomic traits, carotenoid, chlorophyll, and starch content. The expression of five genes related to carotenoid biosynthesis were determined in selected landraces. Analysis revealed a weak negative correlation between starch and β-carotene content, whereas there was a strong positive correlation between root yield and many carotenoids including β-carotene. Carotenoid synthesis genes were expressed in both white and yellow cassava roots, but phytoene synthase 2 (PSY2), lycopene-ε-cyclase (LCYε), and β-carotenoid hydroxylase (CHYβ) expression were generally higher in yellow roots. This study identified lines with reasonably high content of starch and β-carotene that could be candidates for biofortification by further breeding or plant biotechnological means.
    https://dx.doi.org/10.3390/agronomy10030424
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/7439
    IITA Authors ORCID
    Ismail Rabbihttps://orcid.org/0000-0001-9966-2941
    LIVIA STAVOLONEhttps://orcid.org/0000-0002-0691-1302
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.3390/agronomy10030424
    Research Themes
    Biotech and Plant Breeding
    IITA Subjects
    Agronomy; Cassava; Food Security; Plant Breeding; Plant Production
    Agrovoc Terms
    Carotenoids; Biosynthesis; Cassava; Food Security; Vitamin A; Subsaharan Africa; Nigeria
    Regions
    Africa; West Africa
    Countries
    Nigeria
    Hubs
    Headquarters and Western Africa Hub
    Journals
    Agronomy
    Collections
    • Journal and Journal Articles4835
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