Show simple item record

Gene expression and metabolite profiling of thirteen Nigerian cassava landraces to elucidate starch and carotenoid composition

dc.contributor.authorOlayide, P.
dc.contributor.authorLarge, A.
dc.contributor.authorStridh, L.
dc.contributor.authorRabbi, I.
dc.contributor.authorBaldermann, S.
dc.contributor.authorStavolone, L.
dc.contributor.authorAlexandersson, E.
dc.date.accessioned2022-05-05T10:04:06Z
dc.date.available2022-05-05T10:04:06Z
dc.date.issued2020
dc.identifier.citationOlayide, P., Large, A., Stridh, L., Rabbi, I., Baldermann, S., Stavolone, L. & Alexandersson, E. (2020). Gene expression and metabolite profiling of thirteen Nigerian cassava landraces to elucidate starch and carotenoid composition. Agronomy, 10(3), 1-17.
dc.identifier.issn2073-4395
dc.identifier.urihttps://hdl.handle.net/20.500.12478/7439
dc.description.abstractThe prevalence of vitamin A deficiency in sub-Saharan Africa necessitates effective approaches to improve provitamin A content of major staple crops. Cassava holds much promise for food security in sub-Saharan Africa, but a negative correlation between β-carotene, a provitamin A carotenoid, and dry matter content has been reported, which poses a challenge to cassava biofortification by conventional breeding. To identify suitable material for genetic transformation in tissue culture with the overall aim to increase β-carotene and maintain starch content as well as better understand carotenoid composition, root and leaf tissues from thirteen field-grown cassava landraces were analyzed for agronomic traits, carotenoid, chlorophyll, and starch content. The expression of five genes related to carotenoid biosynthesis were determined in selected landraces. Analysis revealed a weak negative correlation between starch and β-carotene content, whereas there was a strong positive correlation between root yield and many carotenoids including β-carotene. Carotenoid synthesis genes were expressed in both white and yellow cassava roots, but phytoene synthase 2 (PSY2), lycopene-ε-cyclase (LCYε), and β-carotenoid hydroxylase (CHYβ) expression were generally higher in yellow roots. This study identified lines with reasonably high content of starch and β-carotene that could be candidates for biofortification by further breeding or plant biotechnological means.
dc.description.sponsorshipVetenskapsrådet
dc.format.extent1-17
dc.language.isoen
dc.subjectCarotenoids
dc.subjectBiosynthesis
dc.subjectCassava
dc.subjectFood Security
dc.subjectVitamin A
dc.subjectSubsaharan Africa
dc.subjectNigeria
dc.titleGene expression and metabolite profiling of thirteen Nigerian cassava landraces to elucidate starch and carotenoid composition
dc.typeJournal Article
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationSwedish University of Agricultural Sciences
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationLeibniz Institute of Vegetable and Ornamental Crops
cg.contributor.affiliationUniversity of Potsdam
cg.contributor.affiliationInstitute for Sustainable Plant Protection, Italy
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.coverage.hubHeadquarters and Western Africa Hub
cg.researchthemeBiotech and Plant Breeding
cg.identifier.bibtexciteidOLAYIDE:2020
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectAgronomy
cg.iitasubjectCassava
cg.iitasubjectFood Security
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Production
cg.journalAgronomy
cg.notesOpen Access Journal; Published online: 20 Mar 2020
cg.accessibilitystatusOpen Access
cg.reviewstatusPeer Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.3390/agronomy10030424
cg.iitaauthor.identifierIsmail Rabbi: 0000-0001-9966-2941
cg.iitaauthor.identifierLIVIA STAVOLONE: 0000-0002-0691-1302
cg.futureupdate.requiredNo
cg.identifier.issue3
cg.identifier.volume10


Files in this item

Thumbnail
Name:
U20ArtOlayideGeneInthomNodev.pdf
Size:
628.9Kb
Format:
PDF
Description:
Journal Article
View/Open

This item appears in the following Collection(s)

Show simple item record