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    CO2 supplementation eliminates sugar-rich media requirement for plant propagation using a simple inexpensive temporary immersion photobioreactor

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    Journal Article (578.0Kb)
    Date
    2022
    Author
    Trauger, M.
    Hile, A.
    Sreenivas, K.
    Shouse, E.M.
    Bhatt, J.
    Lai, T.
    Mohandass, R.
    Tripathi, L.
    Ogden, A.J.
    Curtis, W.R.
    Type
    Journal Article
    Review Status
    Internal Review
    Target Audience
    Scientists
    Metadata
    Show full item record
    Abstract/Description
    In vitro plant propagation systems such as temporary immersion bioreactors (TIBs) are valuable tools that enable production of disease-free plants with improved traits. However, TIB systems can be expensive, difficult to implement, and prone to contamination due to sugar rich propagation media. Using rapidly growing chicory root cultures to expedite design-build-test cycles, we report here an improved, low-cost version of a previously reported Hydrostatically-driven TIB (Hy-TIB) that facilitates economical use of gas mixtures. Bioreactor improvements include decreased material costs, expanded modes of operation, and a horizontal orientation of a plastic film plant growth chambers that increase propagule light exposure. To take advantage of these improvements, we describe here experiments that evaluate the impacts of elevated CO2 on propagation of cacao (Theobroma cacao) secondary embryos and nodal cultures of yam (Dioscorea spp.) during both phototrophic and photomixotrophic growth. Our experiments show that elevated CO2 during plant propagation significantly improved both cacao and yam propagule development and eliminated the need for supplemental sugars in tissue culture growth media. Thus, our improved Hy-TIB shows potential as a simple, low-cost, and scalable propagation platform with cost-effective gas composition control and reduced risk of contamination overgrowth. We provide detailed instructions for assembly of this Hy-TIB design and discuss the implications of its adoption in food-insecure regions of the world.
    https://dx.doi.org/10.1007/s11240-021-02210-3
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/7516
    IITA Authors ORCID
    Leena Tripathihttps://orcid.org/0000-0001-5723-4981
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.1007/s11240-021-02210-3
    Research Themes
    Biotech and Plant Breeding
    IITA Subjects
    Agronomy; Food Security; Genetic Improvement; Plant Breeding; Plant Diseases; Plant Health; Plant Production; Value Chains
    Agrovoc Terms
    Bioreactors; Plant Propagation; Theobroma Cacao; Dioscorea; Food Security; Value Chain
    Countries
    Kenya; Nigeria
    Hubs
    Eastern Africa Hub
    Journals
    Plant Cell, Tissue and Organ Culture
    Collections
    • Journal and Journal Articles4501
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