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CO2 supplementation eliminates sugar-rich media requirement for plant propagation using a simple inexpensive temporary immersion photobioreactor

dc.contributor.authorTrauger, M.
dc.contributor.authorHile, A.
dc.contributor.authorSreenivas, K.
dc.contributor.authorShouse, E.M.
dc.contributor.authorBhatt, J.
dc.contributor.authorLai, T.
dc.contributor.authorMohandass, R.
dc.contributor.authorTripathi, L.
dc.contributor.authorOgden, A.J.
dc.contributor.authorCurtis, W.R.
dc.date.accessioned2022-06-20T15:27:08Z
dc.date.available2022-06-20T15:27:08Z
dc.date.issued2022
dc.identifier.citationTrauger, M., Hile, A., Sreenivas, K., Shouse, E.M., Bhatt, J., Lai, T., ... & Curtis, W.R. (2022). CO2 supplementation eliminates sugar-rich media requirement for plant propagation using a simple inexpensive temporary immersion photobioreactor. Plant Cell, Tissue and Organ Culture, 1-15.
dc.identifier.issn0167-6857
dc.identifier.urihttps://hdl.handle.net/20.500.12478/7516
dc.description.abstractIn vitro plant propagation systems such as temporary immersion bioreactors (TIBs) are valuable tools that enable production of disease-free plants with improved traits. However, TIB systems can be expensive, difficult to implement, and prone to contamination due to sugar rich propagation media. Using rapidly growing chicory root cultures to expedite design-build-test cycles, we report here an improved, low-cost version of a previously reported Hydrostatically-driven TIB (Hy-TIB) that facilitates economical use of gas mixtures. Bioreactor improvements include decreased material costs, expanded modes of operation, and a horizontal orientation of a plastic film plant growth chambers that increase propagule light exposure. To take advantage of these improvements, we describe here experiments that evaluate the impacts of elevated CO2 on propagation of cacao (Theobroma cacao) secondary embryos and nodal cultures of yam (Dioscorea spp.) during both phototrophic and photomixotrophic growth. Our experiments show that elevated CO2 during plant propagation significantly improved both cacao and yam propagule development and eliminated the need for supplemental sugars in tissue culture growth media. Thus, our improved Hy-TIB shows potential as a simple, low-cost, and scalable propagation platform with cost-effective gas composition control and reduced risk of contamination overgrowth. We provide detailed instructions for assembly of this Hy-TIB design and discuss the implications of its adoption in food-insecure regions of the world.
dc.description.sponsorshipNSF BREAD ABRDC
dc.description.sponsorshipBill & Melinda Gates Foundation
dc.format.extent1-15
dc.language.isoen
dc.subjectBioreactors
dc.subjectPlant Propagation
dc.subjectTheobroma Cacao
dc.subjectDioscorea
dc.subjectFood Security
dc.subjectValue Chain
dc.titleCO2 supplementation eliminates sugar-rich media requirement for plant propagation using a simple inexpensive temporary immersion photobioreactor
dc.typeJournal Article
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.affiliationPennsylvania State University
cg.contributor.affiliationSRM University
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.contributor.affiliationPacific Northwest National Laboratories, USA
cg.coverage.countryKenya
cg.coverage.countryNigeria
cg.coverage.hubEastern Africa Hub
cg.researchthemeBiotech and Plant Breeding
cg.identifier.bibtexciteidTRAUGER:2022
cg.isijournalISI Journal
cg.authorship.typesCGIAR and developing country institute
cg.iitasubjectAgronomy
cg.iitasubjectFood Security
cg.iitasubjectGenetic Improvement
cg.iitasubjectPlant Breeding
cg.iitasubjectPlant Diseases
cg.iitasubjectPlant Health
cg.iitasubjectPlant Production
cg.iitasubjectValue Chains
cg.journalPlant Cell, Tissue and Organ Culture
cg.notesOpen Access Article; Published online: 18 Apr 2022
cg.accessibilitystatusOpen Access
cg.reviewstatusInternal Review
cg.usagerightslicenseCreative Commons Attribution 4.0 (CC BY 0.0)
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.1007/s11240-021-02210-3
cg.iitaauthor.identifierLeena Tripathi: 0000-0001-5723-4981
cg.futureupdate.requiredNo


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