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    Mitochondrial coding genome analysis of tropical root-knot nematodes (Meloidogyne) supports haplotype based diagnostics and reveals evidence of recent reticulate evolution

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    U16ArtJanssenMitochondrialInthomNodev.pdf (981.5Kb)
    Date
    2016-03-04
    Author
    Janssen, T.
    Karssen, G.
    Verhaeven, M.
    Coyne, D.L.
    Bert, W.
    Type
    Journal Article
    Target Audience
    Scientists
    Metadata
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    Abstract/Description
    The polyphagous parthenogenetic root-knot nematodes of the genus Meloidogyne are considered to be the most significant nematode pest in sub-tropical and tropical agriculture. Despite the crucial need for correct diagnosis, identification of these pathogens remains problematic. The traditionally used diagnostic strategies, including morphometrics, host-range tests, biochemical and molecular techniques, now appear to be unreliable due to the recently-suggested hybrid origin of root-knot nematodes. In order to determine a suitable barcode region for these pathogens nine quickly-evolving mitochondrial coding genes were screened. Resulting haplotype networks revealed closely related lineages indicating a recent speciation, an anthropogenic-aided distribution through agricultural practices, and evidence for reticulate evolution within M. arenaria. Nonetheless, nucleotide polymorphisms harbor enough variation to distinguish these closely-related lineages. Furthermore, completeness of lineage sorting was verified by screening 80 populations from widespread geographical origins and variable hosts. Importantly, our results indicate that mitochondrial haplotypes are strongly linked and consistent with traditional esterase isozyme patterns, suggesting that different parthenogenetic lineages can be reliably identified using mitochondrial haplotypes. The study indicates that the barcode region Nad5 can reliably identify the major lineages of tropical root-knot nematodes.
    https://dx.doi.org/10.1038/srep22591
    Multi standard citation
    Permanent link to this item
    https://hdl.handle.net/20.500.12478/768
    Digital Object Identifier (DOI)
    https://dx.doi.org/10.1038/srep22591
    IITA Subjects
    Pests Of Plants
    Agrovoc Terms
    Meloidogyne; Nematode Control; Isozymes
    Regions
    Africa; Africa South Of Sahara
    Journals
    Scientific Reports
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    • Journal and Journal Articles4835
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