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dc.contributor.authorJanssen, T.
dc.contributor.authorKarssen, G.
dc.contributor.authorVerhaeven, M.
dc.contributor.authorCoyne, D.L.
dc.contributor.authorBert, W.
dc.date.accessioned2019-12-04T10:57:32Z
dc.date.available2019-12-04T10:57:32Z
dc.date.issued2016-03-04
dc.identifier.citationJanssen, T., Karssen, G., Verhaeven, M., Coyne, D. & Bert, W. (2016). Mitochondrial coding genome analysis of tropical root-knot nematodes (Meloidogyne) supports haplotype based diagnostics and reveals evidence of recent reticulate evolution. Scientific Reports, 6, 22591
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/20.500.12478/768
dc.description.abstractThe polyphagous parthenogenetic root-knot nematodes of the genus Meloidogyne are considered to be the most significant nematode pest in sub-tropical and tropical agriculture. Despite the crucial need for correct diagnosis, identification of these pathogens remains problematic. The traditionally used diagnostic strategies, including morphometrics, host-range tests, biochemical and molecular techniques, now appear to be unreliable due to the recently-suggested hybrid origin of root-knot nematodes. In order to determine a suitable barcode region for these pathogens nine quickly-evolving mitochondrial coding genes were screened. Resulting haplotype networks revealed closely related lineages indicating a recent speciation, an anthropogenic-aided distribution through agricultural practices, and evidence for reticulate evolution within M. arenaria. Nonetheless, nucleotide polymorphisms harbor enough variation to distinguish these closely-related lineages. Furthermore, completeness of lineage sorting was verified by screening 80 populations from widespread geographical origins and variable hosts. Importantly, our results indicate that mitochondrial haplotypes are strongly linked and consistent with traditional esterase isozyme patterns, suggesting that different parthenogenetic lineages can be reliably identified using mitochondrial haplotypes. The study indicates that the barcode region Nad5 can reliably identify the major lineages of tropical root-knot nematodes.
dc.format.extent1-13
dc.language.isoen
dc.subjectMeloidogyne
dc.subjectNematode Control
dc.subjectIsozymes
dc.titleMitochondrial coding genome analysis of tropical root-knot nematodes (Meloidogyne) supports haplotype based diagnostics and reveals evidence of recent reticulate evolution
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.affiliationGhent University
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionAfrica South Of Sahara
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institute
cg.iitasubjectPests Of Plants
cg.journalScientific Reports
cg.howpublishedFormally Published
cg.accessibilitystatusOpen Access
local.dspaceid74472
cg.targetaudienceScientists
cg.identifier.doihttps://dx.doi.org/10.1038/srep22591


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