Striga hermonthica is the most widespread and destructive plant parasite infesting maize and other major crops in sub-Saharan Africa where it causes severe yield losses and threatens food security. Several tolerant maize lines supporting reduced S. hermonthica emergence have been deployed. However, the molecular bases of such resistance are yet poorly understood. Based on a time course comparative gene expression analysis between susceptible and resistant maize lines we have confirmed resistance mechanisms known to be activated upon plant parasite infestation and identified potential novel players worth further investigation e.g. iron homeostasis and mitochondrial respiration-related genes. Most intriguingly, we show a previously unknown strategy of maize post-attachment resistance based on DIMBOA accumulation in S. hermonthica-infested maize roots. S. hermonthica infestation triggers positive regulation of gene expression in the hydroxamic acid (HA) pathway culminating with an accumulation of benzoxazinoids (BX), known for their antifeedant, insecticidal, antimicrobial, and allelopathic activities. We demonstrate that HA root content is positively correlated with S. hermonthica resistance in the resistant parent and its progenies and in unrelated maize lines. Downregulation of HA genes causes increased susceptibility to S. hermonthica infestation in loss-of-function maize mutants. While the mechanism of BX action in parasitic plant resistance is yet to be uncovered, the potential of this discovery for developing effective control and breeding strategies is enormous.

https://doi.org/10.1016/j.plaphy.2023.108134

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Anna Vittoria Carlucciohttps://orcid.org/0000-0001-6887-5792

Nnanna Unachukwuhttps://orcid.org/0000-0003-2701-2477

Melaku Gedilhttps://orcid.org/0000-0002-6258-6014

Abebe Menkirhttps://orcid.org/0000-0002-5907-9177

ANDREAS GISELhttps://orcid.org/0000-0001-7218-9488

LIVIA STAVOLONEhttps://orcid.org/0000-0002-0691-1302

https://doi.org/10.1016/j.plaphy.2023.108134

Biotech and Plant Breeding

Agronomy; Food Security; Maize; Plant Breeding; Plant Production

Hydroxamic Acids; Striga Hermonthica; Maize; Sub-Saharan Africa

Africa; West Africa

Nigeria

Headquarters and Western Africa Hub

Plant Physiology and Biochemistry