Show simple item record

Fine mapping of Msv1, a major QTL for resistance to Maize Streak Virus leads to development of production markers for breeding pipelines

dc.contributor.authorNair, S.K.
dc.contributor.authorBabu, R.
dc.contributor.authorMagorokosho, C.
dc.contributor.authorMahuku, George S.
dc.contributor.authorSemagn, K.
dc.contributor.authorBeyene, Y.
dc.contributor.authorDas, B.
dc.contributor.authorMakumbi, Dan
dc.contributor.authorKumar, P.L.
dc.contributor.authorOlsen, M.
dc.contributor.authorBoddupalli, P.M.
dc.date.accessioned2019-12-04T10:57:59Z
dc.date.available2019-12-04T10:57:59Z
dc.date.issued2015
dc.identifier.citationNair, S.K., Babu, R., Magorokosho, C., Mahuku, G., Semagn, K., Beyene, Y., ... & Boddupalli, P.M. (2015). Fine mapping of Msv1, a major QTL for resistance to Maize Streak Virus leads to development of production markers for breeding pipelines. Theoretical and Applied Genetics, 128(9), 1839-1854.
dc.identifier.issn0040-5752
dc.identifier.urihttps://hdl.handle.net/20.500.12478/888
dc.descriptionPublished online: 17 June 2015
dc.description.abstractAbstract Maize streak virus (MSV) disease is a devastating disease in the Sub-Saharan Africa (SSA), which causes significant yield loss in maize. Resistance to MSV has previously been mapped to a major QTL (Msv1) on chromosome 1 that is germplasm and environment independent and to several minor loci elsewhere in the genome. In this study, Msv1 was fine-mapped through QTL isogenic recombinant strategy using a large F2 population of CML206 × CML312 to an interval of 0.87 cM on chromosome 1. Genome-wide association study was conducted in the DTMA (Drought Tolerant Maize for Africa)-Association mapping panel with 278 tropical/sub-tropical breedinglines from CIMMYT using the high-density genotypingby- sequencing (GBS) markers. This study identified 19 SNPs in the region between 82 and 93 Mb on chromosome 1(B73 RefGen_V2) at a P < 1.00E-04, which coincided with the fine-mapped region of Msv1. Haplotype trend regression identified a haplotype block significantly associated with response to MSV. Three SNPs in this haplotype block at 87 Mb on chromosome 1 had an accuracy of 0.94 in predicting the disease reaction in a collection of breeding lines with known responses to MSV infection. In two biparental populations, selection for resistant Msv1 haplotype demonstrated a reduction of 1.03–1.39 units on a rating scale of 1–5, compared to the susceptible haplotype. High-throughput KASP assays have been developed for these three SNPs to enable routine marker screening in the breeding pipeline for MSV resistanceAbstract Maize streak virus (MSV) disease is a devastating disease in the Sub-Saharan Africa (SSA), which causes significant yield loss in maize. Resistance to MSV has previously been mapped to a major QTL (Msv1) on chromosome 1 that is germplasm and environment independent and to several minor loci elsewhere in the genome. In this study, Msv1 was fine-mapped through QTL isogenic recombinant strategy using a large F2 population of CML206 × CML312 to an interval of 0.87 cM on chromosome 1. Genome-wide association study was conducted in the DTMA (Drought Tolerant Maize for Africa)-Association mapping panel with 278 tropical/sub-tropical breedinglines from CIMMYT using the high-density genotypingby- sequencing (GBS) markers. This study identified 19 SNPs in the region between 82 and 93 Mb on chromosome 1(B73 RefGen_V2) at a P < 1.00E-04, which coincided with the fine-mapped region of Msv1. Haplotype trend regression identified a haplotype block significantly associated with response to MSV. Three SNPs in this haplotype block at 87 Mb on chromosome 1 had an accuracy of 0.94 in predicting the disease reaction in a collection of breeding lines with known responses to MSV infection. In two biparental populations, selection for resistant Msv1 haplotype demonstrated a reduction of 1.03–1.39 units on a rating scale of 1–5, compared to the susceptible haplotype. High-throughput KASP assays have been developed for these three SNPs to enable routine marker screening in the breeding pipeline for MSV resistance
dc.language.isoen
dc.subjectMaize Streak Virus
dc.titleFine mapping of Msv1, a major QTL for resistance to Maize Streak Virus leads to development of production markers for breeding pipelines
dc.typeJournal Article
dc.description.versionPeer Review
cg.contributor.crpMaize
cg.contributor.affiliationInternational Maize and Wheat Improvement Center
cg.contributor.affiliationInternational Institute of Tropical Agriculture
cg.coverage.regionAfrica
cg.coverage.regionWest Africa
cg.coverage.countryNigeria
cg.isijournalISI Journal
cg.authorship.typesCGIAR and advanced research institutes
cg.iitasubjectMaize
cg.journalTheoretical and Applied Genetics
cg.howpublishedFormally Published
cg.accessibilitystatusLimited Access
local.dspaceid76387
cg.identifier.doihttps://dx.doi.org/10.1007/s00122-015-2551-8


Files in this item

Thumbnail
Name:
S15ArtNairMappingInthomNodev.pdf
Size:
1.692Mb
Format:
PDF
View/Open

This item appears in the following Collection(s)

Show simple item record